Test strip detection of Wuchereria bancrofti amplified DNA in wild-caught Culex pipiens and estimation of infection rate by a PoolScreen algorithm
Test strip detection of Wuchereria bancrofti amplified DNA in wild-caught Culex pipiens and estimation of infection rate by a PoolScreen algorithm
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DOI：
10.1046/j.1365-3156.2003.01155.x
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Ramzy, RMR
中科院分区：
文献类型：
--
作者：
Helmy, H;Fischer, P;Ramzy, RMR
班克罗夫特丝虫病是在埃及尼罗河三角洲消灭的目标。需要改进简单的方法来监测蚊媒中的班氏乌切利氏菌感染，从而成功地消除规划。我们评估了SspI-PCR联合DNA检测试纸（TM）法的性能，并使用PoolScreen算法估算蚊虫感染率。在埃及尼罗河三角洲某丝虫病流行村随机抽取的79间房屋共捕获室内静息库蚊769只（24.4%为抗原性血症，8.6%为微丝虫病）。收集的蚊虫采用室内集合，采用SspI-PCR检测。扩增后的寄生虫DNA分别用溴化乙锭（EtBr）染色琼脂糖凝胶电泳和试纸条检测。基于EtBr法和试纸法的PCR检测结果分别为43家（54.4%）和45家（56.9%）。假设1只感染雌蚊/池的最小蚊感染率为6.85%。PoolScreen2算法软件计算的蚊虫感染率为8.1%[95%可信区间5.85,10.47]。因为它更快、更安全，PCR试纸条是一种实用的工具，特别是当它与PoolScreen算法方法结合使用时，用于监测消除规划的成功。
Bancroftian filariasis is targeted for elimination in the Nile Delta of Egypt. Improved simple methods are needed for monitoring Wuchereria bancrofti infection in the mosquito vector and thereby the success of elimination programmes. We evaluated the performance of the SspI-PCR assay combined with a DNA Detection Test Strip(TM) method and used the PoolScreen algorithm method for estimating mosquito infection rates. A total of 769 indoor-resting Culex pipiens were captured in 79 randomly selected houses from a filaria-endemic village in the Nile Delta of Egypt (24.4% antigenaemia and 8.6% microfilaraemia). Collected mosquitoes were pooled by house, and assayed by the SspI-PCR. Amplified parasite DNA was detected by both electrophoresis of agarose gel stained with ethidium bromide (EtBr) and by test strips. PCR based on EtBr and test strip methods identified 43 (54.4%) and 45 (56.9%) houses, respectively, as being filaria positive. The minimum mosquito infection rate, assuming one infected female/pool was 6.85% by the PCR test strips. Mosquito infection rate calculated by the PoolScreen2 algorithm software amounted to 8.1% [95% confidence interval 5.85, 10.47]. Because it is faster and safer, the PCR test strip is a practical tool, especially when combined with the PoolScreen algorithm method, for xenomonitoring the success of elimination programmes.