Molecular characterization of a distinct potyvirus from whitegrass in China
Molecular characterization of a distinct potyvirus from whitegrass in China
复制标题
DOI：
10.1007/s00705-003-0010-3
复制
发表时间：
2003-06
影响因子：
2.7
通讯作者：
Z. Fan;H. Chen;S. Cai;C. Deng;W. Wang;X. Liang;H. Li
中科院分区：
文献类型：
--
作者：
Z. Fan;H. Chen;S. Cai;C. Deng;W. Wang;X. Liang;H. Li
一种分离自多年生禾草（Pennisetum centrasiaticum Tzvel.）在分子水平上进行了表征。测定了该病毒RNA基因组3′端1669 nt的核苷酸序列，包括大核包涵体蛋白（NIb，RNA聚合酶）C端编码区、衣壳蛋白（CP）基因和3′非翻译区（NTR）。CP基因全长909 nt（含终止密码子），编码302个氨基酸，3′端NTR（不含多聚腺苷酸化区）长241 nt。CP的氨基酸序列比较表明，该病毒与高粱花叶病毒和玉米矮花叶病毒的同源性最高，为77%~ 78%，而3′ NTR的氨基酸序列比较表明，该病毒与玉米花叶病毒的同源性最高，为72%。该病毒分离物与马铃薯Y病毒属甘蔗花叶病毒亚组的其他成员在CP氨基酸序列上有一定的亲缘关系。序列的系统发育分析表明，该病毒分离物代表了一个独特的马铃薯Y病毒，并提出了名称狼尾草花叶病毒（PenMV）。
A potyvirus isolated from perennial whitegrass (Pennisetum centrasiaticumTzvel.) in North China was characterized at the molecular level. The 3′ terminal nucleotide (nt) sequence of 1669 nt of the viral RNA genome has been determined, which covered the coding region of the C-terminal part of the large nuclear inclusion protein (NIb, RNA polymerase), capsid protein (CP) gene and the 3′ nontranslated region (NTR). The CP gene consisted of 909 nt (including the stop codon) encoding 302 amino acid residues, and the 3′ NTR was 241 nt in length excluding the polyadenylated tract. Sequence comparison of the amino acids of CPs showed that this virus was most closely related toSorghum mosaic virusandMaize dwarf mosaic viruswith percent identities of 77% to 78% while that of the 3′ NTRs suggested that it was most closely related to Zea mosaic virus with identity of 72%. This virus isolate was to some extent closely related to other members of theSugarcane mosaic virussubgroup of potyviruses for the CP amino acid sequences. Phylogenetic analyses of the sequences indicated that this virus isolate represented a distinct potyvirus, and the name Pennisetum mosaic virus (PenMV) is proposed.