Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep.
Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep.
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DOI：
10.1038/pr.2014.35
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发表时间：
2014-06
影响因子：
3.6
通讯作者：
Jobe AH
中科院分区：
文献类型：
--
作者：
Payne MS;Kemp MW;Kallapur SG;Kannan PS;Saito M;Miura Y;Newnham JP;Stock S;Ireland DJ;Kramer BW;Jobe AH
预防早产和随后的不良新生儿后遗症是我们这个时代最大的临床挑战之一。最近的研究表明念珠菌在早产和胎儿损伤中的作用，因为它们在阴道和/或羊膜腔中定植。我们假设羊膜内白色念珠菌会引起剧烈的急性胎儿炎症反应。单胎妊娠羊在手术分娩前1或2天接受单次羊膜内注射生理盐水（对照组）或107 CFU白色念珠菌，并在妊娠124±2天进行安乐死。白色梭菌在羊膜腔的定植在第1天导致适度的炎症反应，在第2天导致丰富的炎症，其特征是胎儿血小板减少，淋巴细胞减少，以及胎儿膜、皮肤、肺和羊水中炎症细胞因子/趋化因子的显著增加。白色念珠菌在羊膜腔的急性定植引起严重的宫内炎症和胎儿损伤。白色念珠菌是一种强有力的胎儿病原体，可导致不良妊娠结局。
Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesised that intraamniotic C. albicans would cause a vigorous, acute fetal inflammatory response. Sheep carrying singleton pregnancies received single intraamniotic (IA) injections of either saline (control) or 107 CFU C. albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 ± 2 d gestation. Colonization of the amniotic cavity by C. albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterised by fetal thrombocytopenia, lymphopenia and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung and the amniotic fluid. Acute colonization of the amniotic cavity by C. albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen which can contribute to adverse pregnancy outcomes.