Reduced Live Organism Recovery and Lack of Hydrosalpinx in Mice Infected with Plasmid-Free Chlamydia muridarum
Reduced Live Organism Recovery and Lack of Hydrosalpinx in Mice Infected with Plasmid-Free Chlamydia muridarum
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DOI：
10.1128/iai.01543-13
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发表时间：
2014-03-01
影响因子：
3.1
通讯作者：
Zhong, Guangming
中科院分区：
文献类型：
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作者：
Lei, Lei;Chen, Jianlin;Zhong, Guangming
无质粒沙眼衣原体和沙眼衣原体不能引起严重的病理改变。为了评估致病性减弱是否是由于无质粒衣原体的感染不足或无法触发病理反应，我们比较了5个不同品系的小鼠对有能力和无质粒的衣原体的感染情况。所有5株菌均出现输卵管积水，但未接种无质粒的鼠疫杆菌。不含质粒菌诱导的输卵管积水与下生殖道活体恢复率显著降低和上生殖道感染缩短有关。即使通过腔内注射将无质粒菌直接接种到输卵管内，也不能诱导输卵管积水，这伴随着生殖道中无浆菌存活率的显著下降。此外，紫外线灭活后的具有质粒能力的鼠疫杆菌不再能够诱导输卵管积水，即使在高剂量直接进入输卵管时也是如此。综上所述，这些观察结果表明，无质粒的鼠疫杆菌存活率的降低和感染时间的缩短可能是其致病性减弱的重要原因。我们得出结论，足够的输卵管衣原体活体感染可能是导致输卵管积水的必要条件。
Plasmid-free Chlamydia trachomatis and Chlamydia muridarum fail to induce severe pathology. To evaluate whether the attenuated pathogenicity is due to insufficient infection or inability of the plasmidless chlamydial organisms to trigger pathological responses, we compared plasmid-competent and plasmid-free C. muridarum infections in 5 different strains of mice. All 5 strains developed hydrosalpinx following intravaginal inoculation with plasmid-competent, but not inoculation with plasmid-free, C. muridarum. The lack of hydrosalpinx induction by plasmid-free C. muridarum correlated with significantly reduced live organism recovery from the lower genital tract and shortened infection in the upper genital tract. The plasmid-free C. muridarum organisms failed to induce hydrosalpinx even when the organisms were directly inoculated into the oviduct via an intrabursal injection, which was accompanied by significantly reduced survival of the plasmidless organisms in the genital tracts. Furthermore, plasmid-competent C. muridarum organisms after UV inactivation were no longer able to induce hydrosalpinx even when directly delivered into the oviduct at a high dose. Together, these observations suggest that decreased survival of and shortened infection with plasmid-free C. muridarum may contribute significantly to its attenuated pathogenicity. We conclude that adequate live chlamydial infection in the oviduct may be necessary to induce hydrosalpinx.