Gateway-based destination vectors for functional analyses of bacterial ORFeomes:: Application to the min system in Brucella abortus
Gateway-based destination vectors for functional analyses of bacterial ORFeomes:: Application to the min system in Brucella abortus
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DOI：
10.1128/aem.01873-06
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发表时间：
2007-02-01
影响因子：
4.4
通讯作者：
De Bolle, Xavier
中科院分区：
文献类型：
--
作者：
Hallez, Regis;Letesson, Jean-Jacques;De Bolle, Xavier
构建了20个网关兼容的目的载体。该载体包含荧光和表位融合标签、各种药物标记物和复制起点，这应该使它们可用于探索现有的微生物ORF组。在试图验证这些载体中的几个，我们观察到极性和振荡定位的MinD在布鲁氏菌。
Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.