Aging and Diets Regulate the Rat Anterior Pituitary and Hypothalamic Transcriptome
Aging and Diets Regulate the Rat Anterior Pituitary and Hypothalamic Transcriptome
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DOI：
10.1159/000338411
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发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Gaudreau, Pierrette
中科院分区：
文献类型：
--
作者：
Bedard, Karine;Bedard, Julie;Gaudreau, Pierrette
涉及热量限制的饮食干预是预防或延迟与年龄有关的恶化和疾病的有力策略。在几种组织和物种中观察到了它们的有益效果。这项微阵列研究调查了衰老，长期适度热量限制（LTMCR）和长期饮食大豆对20个月大的Sprague-Dawley大鼠垂体前叶和下丘脑基因表达调控的影响。在这两种组织中，衰老调控基因主要涉及细胞防御和修复机制，涉及凋亡、DNA修复、细胞应激、炎症和免疫反应。在衰老的垂体中，上调最高的基因是再生胰岛衍生的3 β（5.77倍），编码一种参与急性应激和炎症的分泌蛋白。LTMCR对35个垂体基因表达的增龄性变化具有保护作用。此外，在垂体中观察到LTMCR对主要参与细胞死亡和细胞应激反应的新调控基因的有益作用。在下丘脑，LTMCR对年龄相关变化的影响是适度的。最后，改变膳食蛋白质的质量（大豆中20%酪蛋白）对两种组织中mRNA水平的调节影响较小。与生长激素功能相关的基因在衰老垂体中也有差异表达。有趣的是，LTMCR阻止了衰老对胰岛素样生长因子结合蛋白-3基因的影响。总之，这项研究提出了新的垂体和下丘脑分子靶点和信号通路，以帮助理解衰老过程和LTMCR的机制。版权所有（C）2012 S. Karger AG，巴塞尔
Dietary interventions involving caloric restriction represent a powerful strategy to prevent or delay age-related deteriorations and diseases. Their beneficial effects have been observed in several tissues and species. This microarray study investigated the effects of aging, long-term moderate caloric restriction (LTMCR) and long-term dietary soy on the regulation of gene expression in the anterior pituitary and hypothalamus of 20-month-old Sprague-Dawley rats. In both tissues, aging regulated genes mainly involved in cell defense and repair mechanisms related to apoptosis, DNA repair, cellular stress, inflammatory and immune response. In the aging pituitary, the highest upregulated gene was the regenerating islet-derived 3 beta (5.77-fold), coding for a secretory protein involved in acute stress and inflammation. A protective effect of LTMCR on age-related change of gene expression was observed for 35 pituitary genes. In addition, beneficial effects of LTMCR in the pituitary were observed on new regulated genes mainly involved in cell death and cell stress response. In the hypothalamus, the effects of LTMCR on age-related changes were modest. Finally, changing the quality of dietary protein (20% casein for soy) had a low impact on the regulation of mRNA levels in both tissues. Genes associated with the somatotroph function were also differentially expressed in the aging pituitary. Interestingly, LTMCR prevented the effect of aging on insulin-like growth factor-binding protein-3 gene. Altogether, this study proposes novel pituitary and hypothalamic molecular targets and signaling pathways to help in understanding the mechanisms involved in aging processes and LTMCR. Copyright (C) 2012 S. Karger AG, Basel