Direct visualization of replication dynamics in early zebrafish embryos
Direct visualization of replication dynamics in early zebrafish embryos
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DOI：
10.1080/09168451.2016.1141039
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发表时间：
2016-05-01
影响因子：
1.6
通讯作者：
Okumura, Katsuzumi
中科院分区：
文献类型：
--
作者：
Kuriya, Kenji;Higashiyama, Eriko;Okumura, Katsuzumi
我们分析了早期斑马鱼胚胎的DNA复制。用胸腺嘧啶类似物5-乙基-2′-脱氧尿苷（EdU）标记整个胚胎的复制DNA，并在单个胚胎来源的细胞中观察复制位点的空间调控。结果揭示了斑马鱼早期胚胎发生过程中非特征的复制动力学。
We analyzed DNA replication in early zebrafish embryos. The replicating DNA of whole embryos was labeled with the thymidine analog 5-ethynyl-2'-deoxyuridine (EdU), and spatial regulation of replication sites was visualized in single embryo-derived cells. The results unveiled uncharacterized replication dynamics during zebrafish early embryogenesis.