The Engineered Chloroplast Genome Just Got Smarter.
The Engineered Chloroplast Genome Just Got Smarter.
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DOI：
10.1016/j.tplants.2015.07.004
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发表时间：
2015-10
影响因子：
20.5
通讯作者：
Daniell H
中科院分区：
文献类型：
--
作者：
Jin S;Daniell H
众所周知，叶绿体通过光合作用提供食物、燃料和氧气来维持地球上的生命。然而，叶绿体基因组也被巧妙地工程化，以赋予有价值的农艺性状和/或作为生物反应器用于生产工业酶，生物制药，生物产品或疫苗。最近在可食用叶中生物药物的超表达方面的突破促进了主要制药公司向临床研究的进展。这篇评论批判性地评估了在开发新的工具，以提高或简化叶绿体中的靶基因的表达进展。这些工具有望进一步开发新型燃料和产品，增强光合作用过程，并增加我们对逆行信号和细胞过程的理解。
Chloroplasts are known to sustain life on earth by providing food, fuel and oxygen through the process of photosynthesis. However, the chloroplast genome has also been smartly engineered to confer valuable agronomic traits and/or serve as bioreactors for production of industrial enzymes, biopharmaceuticals, bio-products or vaccines. The recent breakthrough in hyper-expression of biopharmaceuticals in edible leaves has facilitated the advancement to clinical studies by major pharmaceutical companies. This review critically evaluates progress in developing new tools to enhance or simplify expression of targeted genes in chloroplasts. These tools hold the promise to further the development of novel fuels and products, enhance the photosynthetic process, and increase our understanding of retrograde signaling and cellular processes.