METABOLIC ROLES OF PEPTONE AND YEAST EXTRACT FOR THE CULTURE OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI
METABOLIC ROLES OF PEPTONE AND YEAST EXTRACT FOR THE CULTURE OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI
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DOI：
10.1007/bf01575849
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发表时间：
1991-10-01
期刊：
影响因子：
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通讯作者：
BOUDRANT, J
中科院分区：
文献类型：
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作者：
NANCIB, N;BRANLANT, C;BOUDRANT, J
研究了复合体对含3-磷酸甘油醛脱氢酶基因重组菌生长和产酶的影响。分批培养导致了醋酸盐的积累，这在无酵母膏的培养基中是不被利用的。葡萄糖耗竭后，细胞停止生长，酶活性下降。酵母膏允许醋酸盐的同化和生长，蛋白胨稳定了酶的活性。这两种化合物的加入都能产生最佳的产酶性能。
The influence of complex compounds on the growth of a recombinant strain of Escherichia coli containing the gene encoding glyceraldehyde 3-phosphate dehydrogenase, as well as the production of this enzyme have been studied. Batchwise cultures led to an accumulation of acetate, which was not utilized in a yeast extract-free medium. After glucose exhaustion, growth stopped and enzyme activity decreased. Whereas yeast extract allowed acetate assimilation and growth, peptone stabilized the enzymatic activity. The addition of both compounds resulted in optimal performances for enzyme production.