The component changes of lysophospholipid mediators in colorectal cancer.
The component changes of lysophospholipid mediators in colorectal cancer.
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DOI：
10.1177/1010428319848616
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发表时间：
2019-05-01
期刊：
影响因子：
--
通讯作者：
Ishihara, Soichiro
中科院分区：
文献类型：
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作者：
Kitamura, Chieko;Sonoda, Hirofumi;Ishihara, Soichiro
尽管已知溶血磷脂在多种癌症的发生和进展中发挥重要作用，但其在人类结直肠癌中的作用尚不清楚。在这项研究中，我们的目的是研究结直肠癌组织中的溶血磷脂水平，以鉴定其水平在结直肠癌中发生特异变化的溶血磷脂。我们使用液相色谱-串联质谱法测量了 11 个乙状结肠癌手术标本中癌组织和正常组织中的溶血磷脂水平，因为该领域的最新进展提高了溶血磷脂的检测灵敏度。我们的结果表明，在结肠癌组织中，溶血磷脂酰肌醇和溶血磷脂酰丝氨酸的水平显着高于正常组织（分别为p=0.025和p=0.01），而溶血磷脂酸的水平显着低于正常组织（p=0.0019）。尽管结肠癌组织中溶血磷脂酰甘油的水平高于正常组织，但这种差异并不显着（p=0.11）。脂肪酸分析进一步表明，18:0溶血磷脂酰肌醇和18:0溶血磷脂酰丝氨酸是结肠癌组织中溶血磷脂的主要种类。这些成分可能与结直肠癌的发生有关。
Although lysophospholipids are known to play an important role in the development and progression of several kinds of cancers, their role in human colorectal cancer is as yet unclear. In this study, we aim to investigate lysophospholipid levels in colorectal cancer tissues to identify lysophospholipids, the levels of which change specifically in colorectal cancers. We used liquid chromatography-tandem mass spectrometry to measure lysophospholipid levels in cancerous and normal tissues from 11 surgical specimens of sigmoid colon cancers, since recent advances in this field have improved detection sensitivities for lysophospholipids. Our results indicate that, in colon cancer tissues, levels of lysophosphatidylinositol and lysophosphatidylserine were significantly higher ( p=0.025 and p=0.01, respectively), whereas levels of lysophosphatidic acid were significantly lower ( p=0.0019) than in normal tissues. Although levels of lysophosphatidylglycerol were higher in colon cancer tissues than in normal tissues, this difference was not found to be significant ( p=0.11). Fatty acid analysis further showed that 18:0 lysophosphatidylinositol and 18:0 lysophosphatidylserine were the predominant species of lysophospholipids in colon cancer tissues. These components may be potentially involved in colorectal carcinogenesis.