Postexercise recovery of skeletal muscle malonyl-CoA, acetyl-CoA carboxylase, and AMP-activated protein kinase.
Postexercise recovery of skeletal muscle malonyl-CoA, acetyl-CoA carboxylase, and AMP-activated protein kinase.
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DOI：
10.1152/jappl.1998.85.5.1629
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发表时间：
1998-11
影响因子：
3.3
通讯作者：
Blake B. Rasmussen;Chad R. Hancock;W. Winder
中科院分区：
文献类型：
--
作者：
Blake B. Rasmussen;Chad R. Hancock;W. Winder
先前的研究已经证明，在运动后阶段，氧消耗和脂肪氧化仍然升高。本研究的目的是确定丙二酰辅酶A，脂肪酸氧化的抑制剂，是否在运动后仍然在肌肉中被抑制。将大鼠短跑5 min（40 m/min，5%等级）或跑步30 min（21 m/min，15%等级）。短跑大鼠运动后90分钟红股四头肌丙二酰辅酶A恢复到静息值，30分钟运动组运动后至少90分钟仍显着降低。运动后10 min，两组大鼠AMP活化蛋白激酶活性均显著升高（P < 0.05）。运动后30 min内糖原补充速度最快。在运动30分钟后的90分钟内，呼吸交换率从非运动值0.87 +/-0.01下降到平均0.82 +/- 0.01。因此，肌肉丙二酰辅酶A保持抑制和脂肪氧化升高相对较长的时间后，一轮的运动。这可能使脂肪氧化对肌肉能量需求的贡献更大，从而留下更多的葡萄糖用于补充肌糖原。
Previous studies have demonstrated that oxygen consumption and fat oxidation remain elevated in the postexercise period. The purpose of this study was to determine whether malonyl-CoA, an inhibitor of fatty acid oxidation, remains depressed in muscle after exercise. Rats were sprinted for 5 min (40 m/min, 5% grade) or run for 30 min (21 m/min, 15% grade). Red quadriceps malonyl-CoA returned to resting values by 90 min postexercise in the sprinting rats and remained significantly lower at least 90 min postexercise in the 30-min exercise group. AMP-activated protein kinase activity remained significantly elevated (P < 0.05) for 10 min after exercise in both groups. The most rapid rate of glycogen repletion was in the first 30 min postexercise. The respiratory exchange ratio decreased from a nonexercise value of 0.87 +/- 0.01 to an average 0.82 +/- 0.01 during the 90-min period after 30 min of exercise. Thus muscle malonyl-CoA remains depressed and fat oxidation is elevated for relatively prolonged periods after a single bout of exercise. This may allow fat oxidation to contribute more to muscle energy requirements, thus leaving more glucose for replenishment of muscle glycogen.