Inhibitory effect of genistein on bone resorption in tissue culture
Inhibitory effect of genistein on bone resorption in tissue culture
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DOI：
10.1016/s0006-2952(97)00402-4
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发表时间：
1998-01-01
影响因子：
5.8
通讯作者：
Gao, YH
中科院分区：
文献类型：
--
作者：
Yamaguchi, M;Gao, YH
研究染料木素对体外骨吸收的影响。老年雌性大鼠股骨干骺部组织在添加抗生素和牛血清白蛋白的Dulbecco改良Eagle培养基（高葡萄糖，4.5%）中培养48小时。实验培养含有10(-7)~ 10(-3)M染料木素。骨吸收因子甲状旁腺激素（1-34）（PTH; 10(-7) M）、前列腺素E-2 (PGE(2)；10(-5) M)和脂多糖（10 μ g/mL）使骨钙含量显著降低。染料木素(10（-7)~ 10(-5)M）可完全抑制骨吸收因子引起的骨钙含量下降。此外，这种异黄酮（10(-5)M）完全抑制PTH (10(-7) M)-或PGE(2) (10(-5) M)-诱导的骨组织中葡萄糖消耗和乳酸产生的增加。染料木素（10(-5)M）对PTH （10(-7) M）均有抑制作用，增加骨组织酸性磷酸酶活性，降低骨组织碱性磷酸酶活性。染料木素（10(-5)M）对PTH （10(-7) M）刺激的骨吸收的抑制作用明显被10(-6)M他莫昔芬（一种抗雌激素试剂）的存在所阻止。染料木素（10(-5)M）没有进一步增强雌激素（10(-9)M）对甲状旁腺激素刺激的骨吸收的抑制作用。这些结果表明染料木素在体外组织培养中对骨吸收有直接抑制作用。(C) 1998爱思唯尔科学有限公司
The effect of genistein on bone resorption in vitro was investigated. Femoral-metaphyseal tissues obtained from elderly female rats were cultured for 48 hr in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained 10(-7) to 10(-3) M genistein. The bone-resorbing factors parathyroid hormone (1-34) (PTH; 10(-7) M), prostaglandin E-2 (PGE(2); 10(-5) M), and lipopolysaccharide (10 mu g/mL) caused a significant decrease in bone calcium content. The decrease in bone calcium content induced by bone-resorbing factors was inhibited completely by genistein (10(-7) to 10(-5) M). In addition, this isoflavonoid (10(-5) M) completely inhibited the PTH (10(-7) M)- or PGE(2) (10(-5) M)-induced increase in medium glucose consumption and lactic acid production by bone tissues. Moreover, genistein (10(-5) M) blocked both PTH (10(-7) M)-increased acid phosphatase and -decreased alkaline phosphatase activities of bone tissues. The inhibitory effect of genistein (10(-5) M) on PTH (10(-7) M)-stimulated bone resorption was clearly prevented by the presence of 10(-6) M tamoxifen, an anti-estrogen reagent. Genistein (10(-5) M) did not further enhance the inhibitory effect of estrogen (10(-9) M) on PTH-stimulated bone resorption. These findings indicate that genistein has a direct inhibitory effect on bone resorption in tissue culture in vitro. (C) 1998 Elsevier Science Inc.