In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar
In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar
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DOI：
10.1007/s10735-010-9309-z
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发表时间：
2011-02-01
影响因子：
3.2
通讯作者：
Sakai, Hidetaka
中科院分区：
文献类型：
--
作者：
Honda, Jun-ya;Kobayashi, Ieyoshi;Sakai, Hidetaka
我们先前在胚胎第10.5天(E10.5)的小鼠下颌骨和晚期的E12.0小鼠下颌骨之间进行了cDNA消减，以确定其开始表达的基因。三磷酸腺苷合成酶a亚单位(Atpase6)是E12.0下颌骨包括牙胚中高表达的基因之一。用E10.5~E18.0的小鼠下颌第一磨牙进行原位杂交，以确定Atpase6在牙胚发育过程中的精确表达模式。Atpase6mRNA在E10.5的假想牙上皮和下层间充质中有较强的表达，在E12.0和E13.0的增厚的牙上皮中有较强的表达。在胚胎14.0天的上皮处有较强的原位信号，在15.0天的釉质器官中排除了原始釉质结节的区域。从E16.0到E18.0，Atpase6在宫颈内釉上皮、相邻中间层和外釉上皮均有较强的表达。此外，在发育中的头面部各器官中也同时显示出强的Atpase6信号。这些结果表明，Atpase6与其他头面部器官一样，参与了与牙胚的发生和发育有关的细胞的高能量利用功能。
We previously performed cDNA subtraction between the mouse mandibles on embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to identify genes expressed at its beginning. Adenosine triphosphate synthase subunit a (Atpase6) is one of the highly expressed genes in the E12.0 mandible including tooth germs. In situ hybridization was conducted using the mouse mandibular first molar from E10.5 to E18.0 to determine the precise expression patterns of Atpase6 mRNA in the developing tooth germ. Atpase6 mRNA was strongly expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0 and E13.0. Strong in situ signals were observed in the epithelium at E14.0, and in the enamel organ excluded the area of the primary enamel knot at E15.0. Atpase6 was strongly expressed in the inner enamel epithelium, the adjacent stratum intermedium, and the outer enamel epithelium in the cervical loops from E16.0 to E18.0. In addition, strong Atpase6 signals were coincidently demonstrated in various developing cranio-facial organs. These results suggest that Atpase6 participates in the high energy-utilizing functions of the cells related to the initiation and the development of the tooth germ as well as those of the other cranio-facial organs.