Synthesis of biologically active rat transforming growth factor I
Synthesis of biologically active rat transforming growth factor I
复制标题
具有生物活性的大鼠转化生长因子I的合成
作者：
J. Tam;H. Marquardt;D. Rosberger;T. Wong;G. Todaro
一些转化细胞产生内源性转化生长因子（TGF）的假设得到了从转化细胞中分离肽因子的支持1 -5。一组TGF，TGF-Ⅰ，仅由转化细胞产生，显示与功能相关的小鼠颌下表皮生长因子（mEGF）5的序列同源性。TGF-I和mEGF在竞争结合EGF受体、刺激DNA合成和细胞生长方面表现出相似的活性。存在于正常和转化细胞中的另一组TGF-11（也称为TGFβ）2在结构和功能上与TGF-1或EGF无关，并且不竞争结合EGF受体或诱导细胞生长。然而，TGF-Ⅰ或EGF在TGF-Ⅱ存在下产生协同效应，这是NRK成纤维细胞表型转化的原因4。最近已经确定了来自转化的Fischer大鼠胚胎成纤维细胞的大鼠TGF-1（rTGF-1）的完整氨基酸序列5。使用该序列，我们现在已经通过固相合成方法制备了合成的rTGF-1，并发现它具有与天然rTGF-1相同的化学和生物学性质。由于合成的rTGF-I不含任何生物污染物，我们的研究结果提供了独立的证据，证明rTGF-I是细胞转化中的活性成分6。
The hypothesis that some transformed cells produce endogenous transforming growth factors (TGFs) has been supported by isolation of peptide factors from transformed cells1–5. One group of TGFs, TGF-I, produced only by transformed cells, displays sequence homology with the functionally related mouse submaxillary epidermal growth factor (mEGF)5. Both TGF-I and mEGF exhibit similar activities in competition for binding to the EGF receptor, stimulation of DNA synthesis and cell growth. Another group of TGFs, TGF-II (also known as TGFβ)2, present both in normal and transformed cells, is structurally and functionally unrelated to TGF-I or EGF, and does not compete for binding to the EGF receptor or induce cell growth. However, TGF-I or EGF in the presence of TGF-II produces a synergistic effect that is responsible for the observed phenotypic transformation of NRK fibroblasts4. The complete amino acid sequence of rat TGF-I (rTGF-I) from transformed Fischer rat embryo fibroblasts, has recently been determined5. Using this proposed sequence, we have now prepared synthetic rTGF-I by the solid-phase synthesis method and find that it exhibits chemical and biological properties indistinguishable from those of natural rTGF-I. Since synthetic rTGF-I is free of any biological contaminants, our findings provide independent evidence that rTGF-I is an active principle in the transformation of cells6.
酪氨酰蛋白激酶对血管紧张素类似物的体外磷酸化。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Wong,TW;Goldberg,AR
通讯作者：
Goldberg,AR