Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes
Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes
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DOI：
10.1186/1756-8935-2-2
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发表时间：
2009-02-18
影响因子：
3.9
通讯作者：
Workman, Jerry L.
中科院分区：
文献类型：
--
作者：
Lee, Kenneth K.;Swanson, Selene K.;Workman, Jerry L.
脊髓小脑性共济失调 (SCA) 是一种对身体造成破坏的遗传性疾病，其特征是大脑功能异常，导致协调运动能力逐渐丧失。 SCA 有多种类型，因为有多种基因突变可导致这种疾病。 SCA 类型 1-3、6-10、12 和 17 由 DNA 编码序列中的三核苷酸重复扩展产生。有趣的是，最近的研究表明，SCA7 基因中三核苷酸扩展的增加会导致 SAGA 组蛋白乙酰转移酶复合物的功能缺陷。 SCA7 基因编码 SAGA 复合体的一个亚基。该亚基在酵母中作为 SGF73 基因保守。我们证明 Sgf73 需要将组蛋白去泛素化模块招募到 SAGA 和相关的 SliK(SALSA) 复合体中，并维持组蛋白泛素化水平，这对于许多基因的转录调节是必需的。
Spinocerebellar ataxia (SCA) is a physically devastating, genetically inherited disorder characterized by abnormal brain function that results in the progressive loss of the ability to coordinate movements. There are many types of SCAs as there are various gene mutations that can cause this disease. SCA types 1- 3, 6-10, 12, and 17 result from a trinucleotide repeat expansion in the DNA-coding sequence. Intriguingly, recent work has demonstrated that increased trinucleotde expansions in the SCA7 gene result in defect in the function of the SAGA histone acetyltransferase complex. The SCA7 gene encodes a subunit of the SAGA complex. This subunit is conserved in yeast as the SGF73 gene. We demonstrate that Sgf73 is required to recruit the histone deubiquitination module into both SAGA and the related SliK(SALSA) complex, and to maintain levels of histone ubiquitination, which is necessary for regulation of transcription at a number of genes.