The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli.
The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli.
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SelB 参与大肠杆菌细胞毒性坏死因子 1 的表达。
DOI：
10.1016/j.febslet.2011.05.012
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发表时间：
2011
期刊：
影响因子：
3.5
通讯作者：
Kim,KwangSik
中科院分区：
文献类型：
--
作者：
Yu,Hao;Kim,KwangSik
细胞毒性坏死因子1（CNF1）在脑膜炎致病性大肠杆菌中起重要作用。脑膜炎致病菌E. coliCNF1的表达结果表明，selA和selB基因的转座子突变体不能表达CNF1。我们随后发现，SelB和硒代半胱氨酸，然而，是不是必不可少的CNF1的表达，但SelB在其C末端的47个氨基酸的缺失在翻译水平上CNF1的表达具有显性负效应。生物信息学分析的mRNA ofCNF1预测两个假定的硒代半胱氨酸掺入序列（SECIS）的元素，但我们没有检测到任何硒代半胱氨酸CNF1蛋白。这些发现表明SelB参与CNF1表达的翻译调控，但不参与CNF1蛋白中硒代半胱氨酸的掺入。
Cytotoxic necrotizing factor 1 (CNF1) plays an important role in meningitis-causingEscherichia coli. Mini-Tn5 mutagenesis of meningitis-causingE. colirevealed that transposon mutants ofselAandselBgenes failed to express CNF1. We subsequently showed that SelB and selenocysteine, however, are not essential for the expression of CNF1, but the deletion of 47 amino acids of SelB at its C terminus has a dominant negative effect on CNF1 expression at the translational level. Bioinformatic analysis of the mRNA ofcnf1predicted two putative selenocysteine incorporation sequence (SECIS) elements, but we failed to detect any selenocysteine in CNF1 protein. These findings suggest that SelB is involved in translational regulation of CNF1 expression but without incorporation of selenocysteine in CNF1 protein.