No apoptosis is induced in rat cortical neurons exposed to GSM phone fields
No apoptosis is induced in rat cortical neurons exposed to GSM phone fields
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DOI：
10.1002/bem.20274
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发表时间：
2007-02-01
影响因子：
1.9
通讯作者：
Yardin, Catherine
中科院分区：
文献类型：
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作者：
Joubert, Vanessa;Leveque, Philippe;Yardin, Catherine
本研究旨在探讨射频电磁场(EMF)对体外培养的神经细胞凋亡的影响。将原代培养的胚胎Wistar大鼠大脑皮质神经元暴露于900 MHz全球移动通信系统(GSM)射频场中24小时。使用的平均比吸收率(SAR)为0.25W/kg。用三种方法检测细胞凋亡率：(1)DAPI染色；(2)TdT介导的dUTP缺口末端标记(TUTP)和碘化丙啶(PI)双重染色的流式细胞术；(3)荧光法测定caspase-3活性。无论是在暴露后0h还是在暴露后24 h，对照组和暴露24 h的神经元的凋亡率均无统计学差异。用于评估细胞凋亡的三种方法都是一致的。这些结果表明，在实验所用的条件下，GSM暴露并不能显著增加原代培养的大鼠神经元的凋亡率。这项工作与在细胞系上进行的其他研究一致，据我们所知，这是第一次在培养的皮质神经元上进行的研究。
The aim of this study was to investigate the radiofrequency (RF) electromagnetic fields (EMF) effects on neuronal apoptosis in vitro. Primary cultured neurons from cortices of embryonic Wistar rats were exposed to a 900-MHz global system for mobile communication (GSM) RF field for 24 h in a wire-patch cell. The average-specific absorption rate (SAR) used was 0.25 W/kg. Apoptosis rate was assessed immediately or 24 h after exposure using three methods: (i) DAPI staining; (ii) flow cytometry using double staining with TdT-mediated dUTP nick-end labeling (TUNEL) and propidium iodide (PI); and (iii) measurement of caspase-3 activity by fluorimetry. No statistically significant difference in the apoptosis rate was observed between controls and 24 h GSM-exposed neurons, either 0 h or 24 h post-exposure. All three methods used to assess apoptosis were concordant. These results showed that, under the conditions of experiment used, GSM-exposure does not significantly increase the apoptosis rate in rat primary neuronal cultures. This work is in accordance with other studies performed on cell lines and, to our knowledge, is the first one performed on cultured cortical neurons.