The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys
The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys
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DOI：
10.3389/fphys.2019.01255
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发表时间：
2019-10-11
影响因子：
4
通讯作者：
Bonning, Bryony C.
中科院分区：
文献类型：
--
作者：
Liu, Sijun;Bonning, Bryony C.
褐纹蝽（Halyomorpha halys）是一种入侵性的植食性蝽，在全球农业中具有重要意义。组织特异性转录组学分析的副唾液腺，主唾液腺（PSG）和肠道导致234推定的蛋白酶和166推定的核酸酶序列的鉴定。通过对已报道的H.从唾液中鉴定出22种蛋白酶和2种核酸酶。其中，19种蛋白酶和两种核酸酶存在于WS中。WS中发现的大多数蛋白酶和核酸酶来自PSG，与超微结构观察一致，这表明该组织的活性蛋白质合成和分泌。高转录消化蛋白酶和核酸酶的H。Halys与南方绿色蝽Nezaraviridula的相似，表明这些蝽利用相似的一套蛋白酶和核酸酶消化植物材料。综合数据集的H。这项研究所产生的大比目鱼唾液腺和肠道为进一步了解这种传播有害生物的生物学提供了额外的资源。
The brown marmorated stink bug, Halyomorpha halys, is an invasive, phytophagous stink bug of global importance for agriculture. Tissue-specific transcriptomic analysis of the accessory salivary gland, principal salivary gland (PSG) and gut resulted in identification of 234 putative protease and 166 putative nuclease sequences. By mapping the previously reported proteomes of H. halys watery saliva (WS) and sheath saliva to protein sequences translated from the assembled transcripts, 22 proteases and two nucleases in the saliva were identified. Of these, 19 proteases and both nucleases were present in the WS. The majority of proteases and nucleases found in WS were derived from the PSG, in line with ultrastructural observations, which suggest active protein synthesis and secretion by this tissue. The highly transcribed digestive proteases and nucleases of H. halys were similar to those of the southern green stink bug, Nezara viridula, indicating that these pentatomid stink bugs utilize a similar suite of proteases and nucleases for digestion of plant material. The comprehensive data set for the H. halys salivary glands and gut generated by this study provides an additional resource for further understanding of the biology of this pestiferous species.