Growth of normal and neoplastic mouse mammary epithelial cells in primary culture: stimulation by conditioned medium from mouse mammary fibroblasts.
Growth of normal and neoplastic mouse mammary epithelial cells in primary culture: stimulation by conditioned medium from mouse mammary fibroblasts.
复制标题
原代培养中正常和肿瘤性小鼠乳腺上皮细胞的生长：小鼠乳腺成纤维细胞条件培养基的刺激。
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Mutuyosi Koga
中科院分区：
文献类型：
--
作者：
J. Enami;Sonoko Enami;Mutuyosi Koga
从小鼠乳腺成纤维细胞中获得的条件培养基在小鼠乳腺肿瘤细胞原代单层培养中显著促进DNA合成和细胞增殖。条件培养基同样刺激了雌性小鼠正常乳腺上皮细胞的生长。3T3细胞、3T6细胞、小鼠胚胎成纤维细胞和肺成纤维细胞、乳腺肿瘤细胞和正常乳腺上皮细胞在条件培养基中不存在促生长活性。表皮生长因子（EGF）也能刺激乳腺肿瘤细胞的DNA合成，但效果不如条件培养基。使用125i标记的EGF进行受体研究进一步表明，条件培养基不抑制EGF与乳腺肿瘤细胞的特异性结合。通过Sephadex G-200柱层析，估计乳腺成纤维细胞条件培养基中促生长活性的分子量约为100,000道尔顿。这些观察结果表明，构成乳腺基质的乳腺成纤维细胞产生一种不同于EGF的乳腺上皮细胞生长因子。
Conditioned medium obtained from mouse mammary fibroblasts markedly stimulated both DNA synthesis and cell proliferation in primary monolayer cultures of mouse mammary tumor cells. Growth of normal mammary epithelial cells from virgin female mice was likewise stimulated by the conditioned medium. The growth-stimulating activity was absent in conditioned media from 3T3 cells, 3T6 cells, mouse embryonic and lung fibroblasts, mammary tumor cells and normal mammary epithelial cells. Stimulation of DNA synthesis in mammary tumor cells was also observed with epidermal growth factor (EGF), although the effect was less than that observed with the conditioned medium. Receptor studies using 125I-labeled EGF further showed that the specific binding of EGF to mammary tumor cells was not inhibited by the conditioned medium. The molecular weight of the growth-stimulating activity in mammary fibroblast-conditioned medium was estimated to be approximately 100,000 daltons by Sephadex G-200 column chromatography. These observations suggest that mammary fibroblasts, which constitute the stroma of the mammary gland, produce a mammary epithelial cell growth factor(s) distinct from EGF.
培养的非恶性和恶性人类乳腺上皮细胞的克隆增殖。
影响因子：
11.2
作者：
Smith,HS;Lan,S;Ceriani,R;Hackett,AJ;Stampfer,MR
通讯作者：
Stampfer,MR