Regulated Breathless receptor tyrosine kinase activity required to pattern cell migration area branching in the Drosophila tracheal system
Regulated Breathless receptor tyrosine kinase activity required to pattern cell migration area branching in the Drosophila tracheal system
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DOI：
10.1101/gad.10.22.2912
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发表时间：
1996-11-15
影响因子：
10.5
通讯作者：
Montell, DJ
中科院分区：
文献类型：
--
作者：
Lee, T;Hacohen, N;Montell, DJ
受体酪氨酸激酶(RTK)是一类不同类型的信号分子，因其在细胞命运指定、细胞分化和致癌转化中的作用而闻名。最近，一些RTK被认为与细胞和轴突的运动有关，并且RTK被认为是组织培养细胞的趋化导向因子。我们研究了果蝇成纤维细胞生长因子受体同系物，呼吸(BTL)，它的活性是胚胎气管系统分支形态发生的每个阶段所必需的，是否可能在引导气管细胞的定向迁移中发挥作用。我们发现，在气管发育过程中，一种结构性活性受体的表达干扰了气管细胞的定向迁移，并导致了额外的次级和末端分支形成细胞。内源性BTL信号的减少增强了细胞的迁移缺陷，同时抑制了异位分枝缺陷。这些结果与气管发育模型一致，在该模型中，空间调控的BTL活性滑动气管细胞迁移，而定量调控的BTL活性决定次级和末端分支细胞的命运模式。
Receptor tyrosine kinases (RTKs) are members of a diverse class of signaling molecules well known for their roles in cell fate specification, cell differentiation, and oncogenic transformation. Recently several RTKs have been implicated in cell and axon motility, and RTKs are known to mediate chemotactic guidance of tissue culture cells. We have investigated whether the Drosophila FGF receptor homolog, Breathless (BTL), whose activity is necessary for each phase of branching morphogenesis in the embryonic tracheal system, might play a role in guiding the directed migration of tracheal cells. We found that expression of a constitutively active receptor during tracheal development interfered with directed tracheal cell migration and led to extra secondary and terminal branch-forming cells. Reduction in endogenous BTL signaling enhanced the cell migration defects while suppressing the ectopic branching defects. These results are consistent with a model for tracheal development in which spatially regulated BTL activity slides tracheal cell migration and quantitatively regulated BTL activity determines the patterns of secondary and terminal branching cell fates.