Oxysterol-binding protein (OSBP) enhances replication of intracellular Salmonella and binds the Salmonella SPI-2 effector SseL via its N-terminus
Oxysterol-binding protein (OSBP) enhances replication of intracellular Salmonella and binds the Salmonella SPI-2 effector SseL via its N-terminus
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DOI：
10.1016/j.micinf.2011.09.003
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发表时间：
2012-02-01
影响因子：
5.8
通讯作者：
Finlay, B. Brett
中科院分区：
文献类型：
--
作者：
Auweter, Sigrid D.;Yu, Hong B.;Finlay, B. Brett
被肠沙门氏菌血清型鼠伤寒沙门氏菌转移到宿主细胞中的效应物对细菌的毒力至关重要。对于许多效应器，它们与宿主途径相互作用的机制尚不清楚。我们最近发现了SPI-2效应物SseL与氧甾醇结合蛋白（OSBP）之间的相互作用。我们在这里发现SseL结合OSBP的n端，鼠伤寒沙门氏菌感染导致OSBP的重新分布。我们进一步证明OSBP是胞内鼠伤寒沙门氏菌有效复制所必需的。这表明鼠伤寒沙门氏菌劫持osbp依赖途径以使其细胞内生活方式受益，可能是通过SseL和osbp介导的宿主脂质代谢操纵。(C) 2011年巴斯德研究所。Elsevier Masson SAS出版。版权所有。
Effectors translocated into the host cell by Salmonella enterica serovar Typhimurium are critical for bacterial virulence. For many effectors, the mechanisms of their interactions with host pathways are not yet understood. We have recently found an interaction between the SPI-2 effector SseL and oxysterol-binding protein (OSBP). We show here that SseL binds the N-terminus of OSBP and that S. Typhimurium infection results in redistribution of OSBP. We furthermore demonstrate that OSBP is required for efficient replication of intracellular S. Typhimurium. This suggests that S. Typhimurium hijacks OSBP-dependent pathways to benefit its intracellular life-style, possibly by SseL- and OSBP-mediated manipulation of host lipid metabolism. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.