CULTURE OF HUMAN ENDOTHELIAL CELLS DERIVED FROM UMBILICAL VEINS - IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGICAL CRITERIA
CULTURE OF HUMAN ENDOTHELIAL CELLS DERIVED FROM UMBILICAL VEINS - IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGICAL CRITERIA
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DOI：
10.1172/jci107470
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发表时间：
1973-01-01
影响因子：
15.9
通讯作者：
MINICK, CR
中科院分区：
文献类型：
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作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通过胶原酶消化脐静脉内部，从新鲜获得的人脐带中分离内皮细胞。细胞在组织培养中均一生长5mo，部分细胞传代10代。在细胞生长的对数阶段，细胞倍增时间为92h，光镜、相差显微镜和扫描电子显微镜显示，培养的人内皮细胞生长为单层紧密相对的多边形大细胞，而培养的人成纤维细胞和人血管平滑肌细胞生长为平行排列的细长梭形细胞的重叠层。透射电子显微镜下可见培养的内皮细胞内含有内皮细胞特有的胞浆内含物(Webel-Palade小体)。这些包涵体也存在于脐静脉内皮细胞中，但在培养或原位培养的血管内皮细胞或成纤维细胞中未见。培养的内皮细胞含有丰富的平滑肌肌动球蛋白。培养的内皮细胞也含有与组织供者血型相适应的ABH抗原；这些抗原在培养的平滑肌细胞或成纤维细胞中检测不到。这些研究表明，从形态和免疫方面可识别的人内皮细胞可以培养长达5个月。
Endothelial cells were isolated from freshly obtained human umbilical cords by collagenase digestion of the interior of the umbilical vein. The cells were grown in tissue culture as a homogeneous population for periods up to 5 mo and some lines were subcultured for 10 serial passages. During the logarithmic phase of cell growth, cell-doubling time was 92 h. Light, phase contrast, and scanning electron microscopy demonstrated that cultured human endothelial cells grew as monolayers of closely opposed, polygonal large cells whereas both cultured human fibroblasts and human smooth muscle cells grew as overlapping layers of parallel arrays of slender, spindle-shaped cells. By transmission electron microscopy, cultured endothelial cells were seen to contain cytoplasmic inclusions (Weibel-Palade bodies) characteristic ofin situendothelial cells. These inclusions were also found in endothelial cells lining umbilical veins but were not seen in smooth muscle cells or fibroblasts in culture orin situ. Cultured endothelial cells contained abundant quantities of smooth muscle actomyosin. Cultured endothelial cells also contained ABH antigens appropriate to the tissue donor's blood type; these antigens were not detectable on cultured smooth muscle cells or fibroblasts. These studies demonstrate that it is possible to culture morphologically and immunologically identifiable human endothelial cells for periods up to 5 mo.Images