Intracellular Glutathione Detection Using MnO2-Nanosheet-Modified Upconversion Nanoparticles
Intracellular Glutathione Detection Using MnO2-Nanosheet-Modified Upconversion Nanoparticles
复制标题
DOI：
10.1021/ja2100774
复制
发表时间：
2011-12-21
影响因子：
15
通讯作者：
Liu, Xiaogang
中科院分区：
文献类型：
--
作者：
Deng, Renren;Xie, Xiaoji;Liu, Xiaogang
我们报告了一种新的设计，基于镧系元素掺杂的上转换纳米粒子和二氧化锰纳米片的组合，用于快速，选择性检测谷胱甘肽在水溶液和活细胞中。在这种方法中，在纳米颗粒表面上形成的二氧化锰（MnO2）纳米片用作上转换发光的有效猝灭剂。可以通过引入谷胱甘肽将MnO2还原成Mn2+来开启发光。监测细胞内谷胱甘肽浓度的能力可以合理设计用于靶向药物和基因递送的方便平台。
We report a novel design, based on a combination of lanthanide-doped upconversion nanoparticles and manganese dioxide nanosheets, for rapid, selective detection of glutathione in aqueous solutions and living cells. In this approach, manganese dioxide (MnO2) nanosheets formed on the surface of nanoparticles serve as an efficient quencher for upconverted luminescence. The luminescence can be turned on by introducing glutathione that reduces MnO2 into Mn2+. The ability to monitor the glutathione concentration intracellularly may enable rational design of a convenient platform for targeted drug and gene delivery.