Genetic Architecture of Abdominal Pigmentation in Drosophila melanogaster
Genetic Architecture of Abdominal Pigmentation in Drosophila melanogaster
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DOI：
10.1371/journal.pgen.1005163
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发表时间：
2015-05-01
期刊：
影响因子：
4.5
通讯作者：
Mackay, Trudy F. C.
中科院分区：
文献类型：
--
作者：
Dembeck, Lauren M.;Huang, Wen;Mackay, Trudy F. C.
色素在物种内和物种之间变化，并且通常是适应性的。黑腹果蝇腹部的色素沉着量是一个相对简单的形态特征，它可以作为绘制复杂表型变异遗传基础的模型。在这里，我们评估了自然变异的女性腹部色素沉着的175个测序近交系的果蝇遗传参考面板，来自罗利，NC人口。我们量化了两个最后的腹部节段，背肌5和6（T5，T6）上黑化的比例。我们发现显着的遗传变异的比例黑化和高的广义遗传力为每个term。全基因组关联研究确定了超过150种与T5（84），T6（34）以及T5和T6之间差异（35）的黑化比例相关的DNA变异。与色素变化相关的几个最重要的变异是棕褐色、乌木色和bric-a-brac 1，这些基因已知会影响D。腹部黑腹色素沉着。突变分析和靶向RNAi敲除显示，全基因组关联研究涉及的28个新候选基因中有17个（61%）影响腹部色素沉着。这些基因中的一些涉及发育和调节途径、几丁质产生、角质层结构和囊泡形成和运输。这些研究结果表明，遗传变异可能会影响多个步骤的途径参与terterephalus发展和黑化。这些新的候选者的变异可能作为适应性进化和性选择的目标在D。黑腹菌
Pigmentation varies within and between species and is often adaptive. The amount of pigmentation on the abdomen of Drosophila melanogaster is a relatively simple morphological trait, which serves as a model for mapping the genetic basis of variation in complex phenotypes. Here, we assessed natural variation in female abdominal pigmentation in 175 sequenced inbred lines of the Drosophila melanogaster Genetic Reference Panel, derived from the Raleigh, NC population. We quantified the proportion of melanization on the two most posterior abdominal segments, tergites 5 and 6 (T5, T6). We found significant genetic variation in the proportion of melanization and high broad-sense heritabilities for each tergite. Genome-wide association studies identified over 150 DNA variants associated with the proportion of melanization on T5 (84), T6 (34), and the difference between T5 and T6 (35). Several of the top variants associated with variation in pigmentation are in tan, ebony, and bric-a-brac1, genes known to affect D. melanogaster abdominal pigmentation. Mutational analyses and targeted RNAi-knockdown showed that 17 out of 28 (61%) novel candidate genes implicated by the genome-wide association study affected abdominal pigmentation. Several of these genes are involved in developmental and regulatory pathways, chitin production, cuticle structure, and vesicle formation and transport. These findings show that genetic variation may affect multiple steps in pathways involved in tergite development and melanization. Variation in these novel candidates may serve as targets for adaptive evolution and sexual selection in D. melanogaster.