Transcripts from the Cellular Homologs of Retroviral Oncogenes: Distribution Among Chicken Tissues
Transcripts from the Cellular Homologs of Retroviral Oncogenes: Distribution Among Chicken Tissues
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DOI：
10.1128/mcb.2.6.617-624.1982
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发表时间：
1982-06
影响因子：
5.3
通讯作者：
T. Gonda;D. Sheiness;J. Bishop
中科院分区：
文献类型：
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作者：
T. Gonda;D. Sheiness;J. Bishop
快速转化逆转录病毒的致癌基因（v-onc基因）在正常细胞的基因组中有同源基因（c-onc基因）。在这项研究中，我们在多种鸡细胞和组织中鉴定并定量了四个c-onc基因（c-myb、c-myc、c- erbb和c-src）的转录。聚腺苷化RNA的电泳分析，随后转移到硝化纤维素和杂交克隆的onc探针表明，c-myb， c-myc和c-src各自产生一个成熟的转录本，而c- erbb产生多个转录本（B. Vennstrom和J. M. Bishop, Cell, in press），其丰度在不同的细胞和组织中有所不同。c-myb、c-myc、c- erbb和c-src的转录通过“点印迹”杂交法定量。我们发现c-myc、c- erbb和c-src转录几乎可以在所有被检查的细胞和组织中检测到，而c-myb转录仅在一些造血细胞中检测到；然而，这些细胞属于几个不同的谱系。因此，在任何情况下，c-onc基因的表达都不局限于单个细胞系。c-myb的表达水平与所检查的组织和细胞的造血活性之间似乎存在相关性，这表明c-myb可能主要在未成熟的造血细胞中表达。对携带相应v-onc基因的病毒靶细胞和组织中c-onc RNA水平的检测显示，对特定v-onc基因转化的易感性与同源c-onc基因的表达之间没有明显的直接或反向相关性。
The oncogenes (v-onc genes) of rapidly transforming retroviruses have homologs (c-onc genes) in the genomes of normal cells. In this study, we characterized and quantitated transcription from four c-onc genes, c-myb, c-myc, c-erb, and c-src, in a variety of chicken cells and tissues. Electrophoretic analysis of polyadenylated RNA, followed by transfer to nitrocellulose and hybridization to cloned onc probes showed that c-myb, c-myc, and c-src each give rise to a single mature transcript, whereas c-erb gives rise to multiple transcripts (B. Vennstrom and J. M. Bishop, Cell, in press) which vary in abundance among different cells and tissues. Transcription from c-myb, c-myc, c-erb, and c-src was quantitated by a “dot-blot” hybridization assay. We found that c-myc, c-erb, and c-src transcription could be detected in nearly all cells and tissues examined, whereas c-myb transcription was detected only in some hemopoietic cells; these cells, however, belong to several different lineages. Thus, in no case was expression of a c-onc gene restricted to a single cell lineage. There appeared to be a correlation between levels of c-myb expression and hemopoietic activity of the tissues and cells examined, which suggests that c-myb may be expressed primarily in immature hemopoietic cells. An examination of c-onc RNA levels in target cells and tissues for viruses carrying the corresponding v-onc genes revealed no obvious correlation, direct or inverse, between susceptibility to transformation by a given v-onc gene and expression of the homologous c-onc gene.