HILIC mode separation of polar compounds by monolithic silica capillary columns coated with polyacrylamide
HILIC mode separation of polar compounds by monolithic silica capillary columns coated with polyacrylamide
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DOI：
10.1007/s00216-006-0606-z
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发表时间：
2006-10-01
影响因子：
4.3
通讯作者：
Tanaka, Nobuo
中科院分区：
文献类型：
--
作者：
Ikegami, Tohru;Fujita, Hiroshi;Tanaka, Nobuo
以N-（3-三甲氧基硅丙基）甲基丙烯酰胺为锚基，在硅胶毛细管整体柱上，通过丙烯酰胺的柱上聚合反应制备了HILIC型色谱柱。该产品显示HILIC模式保留特性与三倍更大的渗透性和略高的柱效率相比，市售的酰胺型HILIC柱填充5 μ m的颗粒。的整体硅胶柱的选择性是类似的颗粒柱的各组溶质对核苷，核酸碱基和碳水化合物衍生物，虽然观察到的溶质组的选择性有相当大的差异。虽然基于极性官能团的溶质的保留是小得多的整体硅胶柱，它具有较小的相比，比与颗粒填充柱，前者可以实现更好的分离，利用高渗透性和更高的柱效率的较长的列。
HILIC mode columns were prepared by an on-column polymerization of acrylamide on a monolithic silica capillary column modified with N-(3-trimethoxysilylpropyl)methacrylamide as the anchor group. The products showed HILIC mode retention characteristics with three times greater permeability and slightly higher column efficiency compared to a commercially available amide-type HILIC column packed with 5-mu m particles. The selectivity of the monolithic silica-based column was similar to that of the particulate column for each group of solutes towards nucleosides, nucleic bases and carbohydrate derivatives, although a considerable difference was observed in the selectivity for the solute groups. Although the retention of solutes based on the polar functionality was much smaller with the monolithic silica columns, which had a smaller phase ratio, than with the particle-packed column, the former can achieve better separation utilizing the high permeability and higher column efficiencies of a longer column.