Growth promotion of interspecific hybrid embryos between Cucumis anguria L. and melon (C. melo L.) by fruit heating
Growth promotion of interspecific hybrid embryos between Cucumis anguria L. and melon (C. melo L.) by fruit heating
复制标题
果实加热促进黄瓜与甜瓜种间杂交胚的生长
DOI：
10.1016/j.hpj.2021.11.013
复制
发表时间：
2022
影响因子：
5.7
通讯作者：
Mako Tsunamoto
中科院分区：
文献类型：
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作者：
Yuichi Matsumoto;Mako Tsunamoto
黄瓜属（Cucumis）的野生种，如C. anguria，对各种甜瓜（C.梅洛）病害如枯萎病1.2号生理小种，并预期作为甜瓜的遗传资源。但在种间杂交中，C.甜瓜和甜瓜的胚在生长早期就败育，即使培养也不能再生。因此，对于胚胎挽救，在流产发生期间促进胚胎生长是必要的。研究了果实加热对柑橘种间杂种胚生长的影响。安瓜和甜瓜。在加热装置中，果实周围的夜间最低温度保持在21.5 ℃以上。C.胚珠粒面积的变化趋势与C.与不加热果实相比，加热果实的anguria× MR-1的果实体积显著增大。与C.安哥拉自花授粉。但是，在C。anguria× MR-1授粉后10 d才观察到胚，授粉后13 d观察到前胚。相反，在C。anguria× MR-1在10 DAP时甚至观察到前胚。这些结果表明，由于胚生长促进，胚或胚珠培养再生的可能性增加。
Wild species of the genusCucumis, such asC. anguria, are resistant to various melon (C. melo) diseases such as Fusarium wilt race 1.2 and are anticipated as genetic resources of melon. However, in the interspecific crosses betweenC. anguriaand melon, the abortion of the embryos was observed in the early growth stage and could not be regenerated even when cultured. Therefore, for embryo rescue, it is necessary to promote embryo growth during abortion occurrence. This study investigated the effects of fruit heating on embryo growth in interspecific hybrids ofC. anguriaand melon. In the heating device, the minimum night temperature around the fruit was maintained above 21.5 °C. The ovule grain area ofC. anguria× MR-1 with fruit heating was significantly enlarged compared to that without fruit-heating. It was also significantly enlarged compared with the ovule grain area ofC. anguriaself-pollination. Although, inC. anguria× MR-1 without fruit-heating, no embryo was observed until 10 days after pollination (DAP), and pre-embryos were observed at 13 DAP. Conversely, inC. anguria× MR-1 with fruit-heating, pre-embryos were even observed at 10 DAP. These results suggest that the possibility of embryo or ovule culture regeneration has increased due to embryo growth promotion.