DIET, OVERFEEDING, AND MODERATE DIETARY RESTRICTION IN CONTROL SPRAGUE-DAWLEY RATS .2. EFFECTS ON AGE-RELATED PROLIFERATIVE AND DEGENERATIVE LESIONS
DIET, OVERFEEDING, AND MODERATE DIETARY RESTRICTION IN CONTROL SPRAGUE-DAWLEY RATS .2. EFFECTS ON AGE-RELATED PROLIFERATIVE AND DEGENERATIVE LESIONS
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DOI：
10.1177/019262339502300306
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发表时间：
1995-05-01
影响因子：
1.5
通讯作者：
CLARK, RL
中科院分区：
文献类型：
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作者：
KEENAN, KP;SOPER, KA;CLARK, RL
本研究比较了2种不同饮食的随意（AL）过度喂养和适度饮食限制（DR）对Sprague-Dawley（SD）大鼠存活率和自发的、年龄相关的增殖性和退行性病变的影响。SD大鼠喂食Purina Rodent Chow 5002或改良的Rodent Chow 5002-9，其含有较低的蛋白质、脂肪、代谢能，并通过AL或通过DR以65%的AL量通过测量或时间（6.5小时）增加纤维。在106周时，喂食5002-9饲料AL的大鼠与喂食5002饲料AL的大鼠相比，存活率没有显著提高。喂食DR的5002饲料随时间（6.5小时）改善了雄性大鼠的存活率，但对雌性大鼠没有改善。只有通过测量两种饮食的DR导致两种性别的死亡率较低。到106周，通过AL喂食任一饮食的大鼠具有与DR喂食的大鼠相同的脑重量，但是AL喂食的大鼠具有更大的体重、体脂含量和增加的心脏、肺、肾、肝、肾上腺、甲状腺和垂体重量，这与这些器官中退行性和/或增殖性病变的发生率和严重程度增加相关。中度DR通过延迟肾小球肥大的早期发展来延迟慢性肾病的进展，肾小球肥大在AL过度喂养大鼠中启动肾小球硬化和肾单位丢失的发展。中度DR降低了心肌病和其他退行性、年龄相关病变的发生率、严重程度和进展，并似乎延缓了SD雌性动物生殖衰老的发展。本研究的结论是，中度DR延迟了退行性病变的发生和进展，以及心血管或肾脏疾病导致的死亡，从而可能改善生物测定以检测化合物特异性慢性毒性。
This study compared the effects of ad libitum (AL) overfeeding and moderate dietary restriction (DR) of 2 different diets on Sprague-Dawley (SD) rat survival and spontaneous, age-related proliferative and degenerative lesions. SD rats were fed Purina Rodent Chow 5002 or a modified Rodent Chow 5002-9 containing lower protein, fat, metabolizable energy, and increased fiber by AL or by DR at 65% of the AL amount by measurement or time (6.5 hr). At 106 wk, rats fed the 5002-9 diet AL did not have significantly improved survival over rats fed the 5002 diet AL. The 5002 diet fed DR by time (6.5 hr) improved survival for males but not females. Only DR by measurement of both diets resulted in lower mortality for both sexes. By 106 wk rats fed either diet by AL had the same brain weights as DR fed rats, but AL fed rats had greater body weight, body fat content, and increased heart, lung, kidney, liver, adrenal, thyroid, and pituitary weights that correlated with an increased incidence and severity of degenerative and/or proliferative lesions in these organs. Moderate DR delayed the progression of chronic nephropathy by delaying the early development of glomerular hypertrophy that initiates the development of glomerular sclerosis and nephron loss in AL overfed rats. Moderate DR lowered the incidence, severity, and progression of cardiomyopathy and other degenerative, age-related lesions and appeared to delay the development of reproductive senescence in SD females. The conclusion from this study is that moderate DR delayed onset and progression of degenerative lesions, and death due to cardiovascular or renal disease, and thus potentially improves the bioassay to detect compound-specific chronic toxicity.