Rabbit antibodies against the human milk sialyloligosaccharide alditol of LS-tetrasaccharide a (NeuAc alpha 2-3Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4GlcOH).
Rabbit antibodies against the human milk sialyloligosaccharide alditol of LS-tetrasaccharide a (NeuAc alpha 2-3Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4GlcOH).
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针对 LS-四糖 a 的人乳唾液酸寡糖醛醇 (NeuAc α 2-3Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4GlcOH) 的兔抗体。
DOI：
10.1016/0003-9861(85)90386-8
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发表时间：
1985
影响因子：
3.9
通讯作者：
Torres,BV
中科院分区：
文献类型：
--
作者：
Smith,DF;Prieto,PA;Torres,BV
The sialyloligosaccharide, NeuAcα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc (LS-tetrasaccharidea), a minor component of human milk, is obtained in relatively large quantities from autohydrolysates of the major milk disialyloligosaccharide, NeuAcα2-3Galβ1-3[NeuAcα2-6]GlcNAcβ1-3Galβ1-4Glc (disialyllacto-N-tetraose). Rabbits immunized with an oligosaccharide-protein conjugate prepared from keyhole limpet hemocyanin and LS-tetrasaccharideaproduce antibodies directed against the corresponding oligosaccharide alditol. The anti-LS-tetrasaccharideasera bind3H-labeled LS-tetrasaccharideain a direct-binding radioimmunoassay on nitrocellulose filters. The specificities of these antibodies are determined by comparing inhibitory activities of structurally related oligosaccharides. Strong hapten-antibody binding (Ka> 106m−1) requires sialic acid linked α2–3 to the nonreducing terminal galactose residue of reduced lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4GlcOH). Specificities of antibodies prepared against keyhole limpet hemocyanin conjugates of LS-tetrasaccharideb(Galβ1-3[NeuAcα2-6]GlcNAcβ1-3Galβ1-4Glc) and LS-tetrasaccharidec(NeuAcα2-6Galβ1-4GlcNAcβ1-3Galβ1-4Glc) differ only slightly from rabbit antibodies prepared against the corresponding bovine serum albumin conjugates described previously [D. F. Smith and V. Ginsburg (1980)J. Biol. Chem.255, 55–59].
