MAO inhibitors and oxidant stress in aging brain tissue
MAO inhibitors and oxidant stress in aging brain tissue
复制标题
DOI：
10.1016/s0924-977x(98)00035-2
复制
发表时间：
1999-03-01
影响因子：
5.6
通讯作者：
Ersöz, B
中科院分区：
文献类型：
--
作者：
Alper, G;Girgin, FK;Ersöz, B
衰老的过程本身表现为生理事件的各种变化。在众多的衰老学说中，自由基衰老学说反映了自由基对细胞成分的损伤，是公认的最重要的衰老学说之一。近年来，衰老过程中儿茶酚胺代谢的增加也引起了人们的关注，单胺氧化酶（MAO）作为这一过程中的关键酶也得到了广泛的研究。本研究的目的是通过测定脂质过氧化产物（LPP）来评估FR物种通过MAO（FR的可能来源）在生理衰老中的作用（丙二醛，二烯共轭物）和抗氧化酶水平（超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在年轻（3月龄，n=10）和老化（16-18月龄，n=10）瑞士雄性白化病大鼠的大鼠脑组织。在研究的第二部分中，在急性给予MAO抑制剂（deprenyl和帕吉林，25 mg/kg腹腔注射）后确定了相同的参数以研究这些试剂是否在通过抑制MAO减少氧化应激中具有任何有益作用。在老年大鼠脑中，随着年龄的增长，MAO活性显著增加（P=0.000），LPP无显著增加，而SOD（P=0.007）和CAT活性则呈下降趋势。在急性给予Deprenyl和帕吉林后，观察到年轻大鼠（P=0.0002）和老年大鼠（P = 0.0002，Deprenyl和P=0.0001，帕吉林）的MAO活性显著降低。结果表明，丙炔苯丙胺能显著提高CAT活性（P
The process of aging presents itself with various alterations in physiological events. Among many theories, the free radical (FR) theory of aging which reflects the FR damage to cellular components is accepted as one of the most important theories. Recently, the increases in catecholamine metabolism in aging have also attracted attention, and monoamine oxidase (MAO), a key enzyme in this process has been extensively studied. The aim of this study was to assess the role of FR species via MAO, a possible source of FRs, in physiological aging by determining the lipid peroxidation products (LPP) (malondialdehyde, diene conjugates) and antioxidant enzyme levels (superoxide dismutase (SOD) and catalase (CAT) in young (3 months old, n=10) and aging (16-18 months old, n=10) rat brain tissues of Swiss male albino rats. In the second part of the study, the same parameters were determined after the acute administration of MAO inhibitors (deprenyl and pargyline, 25 mg/kg i.p.) to investigate whether these agents have any beneficial effects in reducing oxidant stress via inhibition of MAO. In old rat brains, MAO activities showed a significant increase (P=0.000) in addition to an insignificant increase in LPP, while SOD (P=0.007) and CAT activities showed a decrease with advancing age. After the acute administration of both deprenyl and pargyline, a significant decrease in the MAO activities of both young (P=0.0002 for each) and aging rats (P=0.0002 for deprenyl and P=0.0001 for pargyline) were observed. It was noted that deprenyl causes a significant increase in CAT activity (P