Diatom-Inspired Silica Nanostructure Coatings with Controllable Microroughness Using an Engineered Mussel Protein Glue to Accelerate Bone Growth on Titanium-Based Implants
Diatom-Inspired Silica Nanostructure Coatings with Controllable Microroughness Using an Engineered Mussel Protein Glue to Accelerate Bone Growth on Titanium-Based Implants
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DOI：
10.1002/adma.201704906
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发表时间：
2017-12-13
影响因子：
29.4
通讯作者：
Cha, Hyung Joon
中科院分区：
文献类型：
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作者：
Jo, Yun Kee;Choi, Bong-Hyuk;Cha, Hyung Joon
二氧化硅纳米颗粒(SiNPs)已被用于构建生物活性纳米结构，包括表面形貌特征和生物活性，增强骨细胞在钛基种植体上的活性。然而，以前还没有人尝试基于SiNP纳米结构来创建微粗糙表面，尽管微粗糙度被确定为在改善钛植入物的生物力学互锁方面提供有益效果的特征。在此，一种基于蛋白质的SiNP涂层被提出作为一种促进骨表面功能化的方法来在钛种植体表面产生微观粗糙度。生物工程重组贻贝黏附蛋白与硅胶沉淀R5肽(R5-MAP)融合，可以在温和的条件下通过SiNP纳米结构的多层组装直接控制表面的微观粗糙度。组装的SiNP纳米结构以粗糙度依赖的方式显着增强前成骨细胞的体外成骨细胞行为，并促进颅骨缺损处钛种植体上的体内骨组织形成。因此，基于R5-MAP的SiNP纳米结构组装体可以实际应用于加速骨组织生长，提高医用植入设备的稳定性，延长其使用寿命。
Silica nanoparticles (SiNPs) have been utilized to construct bioactive nanostructures comprising surface topographic features and bioactivity that enhances the activity of bone cells onto titanium-based implants. However, there have been no previous attempts to create microrough surfaces based on SiNP nanostructures even though microroughness is established as a characteristic that provides beneficial effects in improving the biomechanical interlocking of titanium implants. Herein, a protein-based SiNP coating is proposed as an osteopromotive surface functionalization approach to create microroughness on titanium implant surfaces. A bioengineered recombinant mussel adhesive protein fused with a silica-precipitating R5 peptide (R5-MAP) enables direct control of the microroughness of the surface through the multilayer assembly of SiNP nanostructures under mild conditions. The assembled SiNP nanostructure significantly enhances the in vitro osteogenic cellular behaviors of preosteoblasts in a roughness-dependent manner and promotes the in vivo bone tissue formation on a titanium implant within a calvarial defect site. Thus, the R5-MAP-based SiNP nanostructure assembly could be practically applied to accelerate bone-tissue growth to improve the stability and prolong the lifetime of medical implantable devices.