Matrix metalloproteinase-2 and-9 differentially regulate smooth muscle cell migration and cell-mediated collagen organization
Matrix metalloproteinase-2 and-9 differentially regulate smooth muscle cell migration and cell-mediated collagen organization
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DOI：
10.1161/01.atv.0000100402.69997.c3
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发表时间：
2004-01-01
影响因子：
8.7
通讯作者：
Galis, ZS
中科院分区：
文献类型：
--
作者：
Johnson, C;Galis, ZS
目的：平滑肌细胞（SMC）产生基质金属蛋白酶（MMP）-2和MMP-9，这两种酶具有相似的体外基质降解能力。我们比较了这些酶的具体贡献SMC-基质相互作用在体外和在vivo.Methods和结果使用遗传模型的缺陷，我们研究了MMP-2和MMP-9的作用，在SMC迁移在体内形成的内膜增生和体外。此外，我们研究了MMP-2和MMP-9基因缺陷对SMC胶原致密化和组装的潜在影响。
Objectives-Smooth muscle cells (SMCs) produce both matrix metalloproteinase (MMP)-2 and MMP-9, enzymes with similar in vitro matrix degrading abilities. We compared the specific contributions of these enzymes to SMC-matrix interactions in vitro and in vivo.Methods and Results-Using genetic models of deficiency, we investigated MMP-2 and MMP-9 roles in SMC migration in vivo in the formation of intimal hyperplasia and in vitro. In addition, we investigated potential effects of MMP-2 and MMP-9 genetic deficiency on compaction and assembly of collagen by SMCs.Conclusions-MMP-2 and MMP-9 genetic deficiency decreased by 81% and 65%, respectively (P