Structures of sugar chains of Ricinus communis agglutinin
Structures of sugar chains of Ricinus communis agglutinin
复制标题
蓖麻凝集素糖链结构
DOI：
10.1016/0304-4165(88)90118-3
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
G. Funatsu
中科院分区：
文献类型：
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作者：
Y. Kimura;S. Hase;Yuji Kobayashi;Y. Kyōgoku;T. Ikenaka;G. Funatsu
测定了蓖麻凝集素糖链的结构。根据公开的方法（Kimura，Y.和Funatsu，G.（1988）Agric.Biol.Chem.52，出版中），并通过肼解释放各糖肽的糖链。将游离氨基N-乙酰化，并将还原末端残基与2-氨基吡啶偶联。通过凝胶过滤和反相HPLC纯化所得的糖链的吡啶基氨基衍生物。由此纯化的PA-糖链的结构通过组分分析、逐步外切糖苷酶二聚化、部分乙酰解和500 MHz 1H-NMR光谱的组合来确定。这些结果表明R. communisagglutinin含有249页所示的糖链。
The structures of sugar chains fromRicinus communisagglutinin were determined. Four glycopeptides were isolated from the lectin according to a published method (Kimura, Y. and Funatsu, G. (1988) Agric. Biol. Chem. 52, in press), and sugar chains of each glycopeptide were liberated by hydrazinolysis. Free amino groups wereN-acetylated and the reducing-end residues were coupled with 2-aminopyridine. The resulting pyridylamino derivatives of sugar chains were purified by gel filtration and reversed-phase HPLC. The structures of thus-purified PA-sugar chains were determined by a combination of component analysis, stepwise exoglycosidase digestions, partial acetolysis, and 500 MHz1H-NMR spectroscopy. These results indicate thatR. communisagglutinin contains the sugar chains shown on page 249.
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