Selective mutagenic activation by cytochrome P3-450 of carcinogenic arylamines found in foods.
Selective mutagenic activation by cytochrome P3-450 of carcinogenic arylamines found in foods.
复制标题
细胞色素 P3-450 对食品中致癌芳胺的选择性诱变激活。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
N. Battula
中科院分区：
文献类型：
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作者：
E. Snyderwine;N. Battula
鱼和牛肉等熟食中发现的杂环芳胺是强效诱变剂和致癌剂。本研究的目的是确定细胞色素 P1-450 和 P3-450 对这些芳胺代谢激活的特异性。我们使用了一种新型致突变性测试系统，该系统将表达重组细胞色素 P1-450 或 P3-450 的人类细胞与鼠伤寒沙门氏菌结合起来，对突变体进行评分。细胞色素 P3-450 是细胞色素 P-450 超基因家族的单一亚型，可生物激活这些食物诱变剂。细胞色素 P1-450 显示很少或没有这些芳胺的活化，但其异构体主要负责芳香烃苯并[a]芘-7,8-二醇的活化。该测定系统应用于定义单个细胞色素 P-450 在致癌物代谢激活中的特异性。
Heterocyclic arylamines found in cooked foods including fish and beef are potent mutagens and carcinogens. The purpose of this investigation was to determine the specificity of cytochromes P1-450 and P3-450 toward the metabolic activation of these arylamines. We used a novel mutagenicity test system which combined human cells expressing either recombinant cytochrome P1-450 or P3-450 with Salmonella typhimurium to score mutants. Cytochrome P3-450, a single isoform of the cytochrome P-450 supergene family, bioactivated these food mutagens. Cytochrome P1-450 showed little or no activation of these arylamines but was the isoform predominantly responsible for the activation of the aromatic hydrocarbon benzo[a]pyrene-7,8-diol. This assay system should serve to define the specificities of individual cytochromes P-450 in the metabolic activation of carcinogens.