Invariant NKT cells rapidly activated via immunization with diverse contact antigens collaborate in vitro with B-1 cells to initiate contact sensitivity
Invariant NKT cells rapidly activated via immunization with diverse contact antigens collaborate in vitro with B-1 cells to initiate contact sensitivity
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DOI：
10.4049/jimmunol.177.6.3686
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发表时间：
2006-09-15
影响因子：
4.4
通讯作者：
Askenase, Philip W.
中科院分区：
文献类型：
--
作者：
Campos, Regis A.;Szczepanik, Marian;Askenase, Philip W.
在皮肤接触敏感性中，存在通过 IgM Ab 激活的早期引发的补体、肥大细胞和血小板的先天级联反应。这种反应是通过导致效应 T 细胞的局部募集来引发获得性经典接触敏感性所必需的。我们最近进行的体内实验表明，先天性不变 V α 14(+) NKT 细胞 (iNKT) 和先天性 B-1 B 细胞亚群之间需要协作才能诱导这一启动过程。接触致敏触发 iNKT 细胞产生 IL-4，与特定 Ag 一起共同激活 B-1 细胞，从而产生起始 IgM Ab。我们现在描述 iNKT 和 B-1 细胞的体外协作。正常腹膜 B-I 细胞在体外与可溶性 Ag 一起孵育，并与体内产生 IL-4 的免疫 iNKT 细胞一起孵育 1 小时，被激活以介导接触敏感性启动级联。该过程的三个组成部分可以被不同的Ag激活。因此，用三种不同的Ag敏化可诱导1小时iNKT细胞激活、B-1细胞刺激和免疫效应T细胞的产生，分别产生IL-4和Ag特异性IgM Ab，以招募Ag特异性效应T细胞。这些发现与感染可能引发的过敏和自身免疫性疾病有关。 T 细胞对过敏原或自身抗原的反应加剧。
In cutaneous contact sensitivity there is an early elicited innate cascade of complement, mast cells, and platelets activated via IgM Abs. This response is required to initiate the elicitation of acquired classical contact sensitivity by leading to local recruitment of effector T cells. We recently performed in vivo experiments showing that collaboration is required between innate-like invariant V alpha 14(+) NKT cells (iNKT) and the innate-like B-1 B cell subset to induce this initiation process. Contact sensitization triggers iNKT cells to produce IL-4 to coactivate the B-1 cells along with specific Ag for production of the initiating IgM Abs. We now describe in vitro collaboration of iNKT and B-1 cells. Normal peritoneal B-I cells, incubated in vitro with soluble Ag, and with 1-h in vivo immune iNKT cells producing IL-4, are activated to mediate the contact sensitivity-initiation cascade. The three components of this process can be activated by different Ag. Thus, 1-h iNKT cell activation, B-1 cell stimulation, and generation of immune effector T cells can be induced by sensitization with three different Ag to respectively generate IL-4 and Ag-specific IgM Abs, to recruit the Ag-specific effector T cells. These findings have relevance to-allergic and autoimmune diseases in which infections can trigger. exacerbation of T cell responses to allergens or to autoantigens.