Amino acid metabolism and inflammatory burden in ovarian cancer patients undergoing intense oncological therapy
Amino acid metabolism and inflammatory burden in ovarian cancer patients undergoing intense oncological therapy
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DOI：
10.1016/j.clnu.2007.07.004
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发表时间：
2007-12-01
影响因子：
6.3
通讯作者：
Sheffield-Moore, Melinda
中科院分区：
文献类型：
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作者：
Dillon, E. L.;Volpi, Elena;Sheffield-Moore, Melinda
背景和目标：癌症和肿瘤治疗与进行性身体恶化、营养不良和炎症负荷增加有关。我们的大量数据显示氨基酸（AA）具有强大的合成代谢潜力，这使我们测试AA是否可以急性刺激接受强烈化疗的癌症患者（CA）的肌肉蛋白质合成。方法：混合肌合成分数（FSR）、苯丙氨酸出现率和消失率在预充的L-[环-H-2（5）]苯丙氨酸的恒定输注期间测量（Ra和Rd）和净苯丙氨酸平衡（NB）。在基础状态下和在每10分钟给予30 mL推注40 g AA 3 h后采集血液和肌肉组织样品。结果：CA患者骨骼肌TNF-α、IL-6和NF-κ B水平升高，与正常对照组比较，差异有统计学意义（P < 0. 05）。FSR和模型衍生的蛋白质合成（Rd）从基础到AA显著增加（FSR：0.052+/-0.009 vs. 0.120+/-0.008%h（-1），P
Background & aims: Cancer and oncological therapy are associated with a progressive physical deterioration, malnutrition, and enhanced inflammatory burden. Our considerable data showing the strong anabolic potential of amino acids (AA) led us to test whether AA can acutely stimulate muscle protein synthesis in cancer patients (CA) undergoing intense chemotherapy.Methods: Mixed muscle fractional synthesis rate (FSR), rates of phenylatanine appearance and disappearance (Ra and Rd), and net phenylatanine balance (NB) were measured during a primed constant infusion of L-[ring-H-2(5)]phenylalanine. Blood and muscle tissue samples were collected in the basal state and following ingestion of 40g of AA given in 30mL boluses every 10 min for 3 h. Serum and tissue cytokines and NF-kappa B expression in skeletal muscle were measured and compared to normative, healthy older controls (OC).Results: Skeletal muscle TNF-alpha, IL-6, and NF-kappa B were elevated in CA. FSR and model-derived protein synthesis (Rd) increased significantly from basal to AA (FSR: 0.052+/-0.009 vs. 0.120+/-0.008%h(-1), P