Act1, an NF-κB-activating protein
Act1, an NF-κB-activating protein
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DOI：
10.1073/pnas.160265197
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发表时间：
2000-09-12
影响因子：
11.1
通讯作者：
Stark, GR
中科院分区：
文献类型：
--
作者：
Li, XX;Commane, M;Stark, GR
使用NF-κ B依赖性选择标记促进了含有编码NF-κ B激活剂Act 1的cDNA的细胞系的分离。Act 1与I κ B激酶（IKK）结合并激活，导致NF-κ B从其与I κ B的复合物中释放。许多释放NF-κ B的信号通路也通过Jun激酶（JNK）激活转录激活因子（ATF）和激活蛋白1（AP-1）。Act 1也激活JNK，这表明它可能是参与NF-κ B和JNK激活的多功能复合物的一部分。Act 1不能激活IL-1无应答突变细胞系中的NF-κ B，在该细胞系中存在所有已知的信号传导组分，这表明它与IL-1信号传导中的未知组分相互作用。
Use of an NF-kappa B-dependent selectable marker facilitated the isolation of a cell line containing a cDNA encoding Act1, an NF-kappa B activator. Act1 associates with and activates I kappa B kinase (IKK), leading to the liberation of NF-kappa B from its complex with I kappa B. Many signaling pathways that liberate NF-kappa B also activate activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). Act1 also activates JNK, suggesting that it might be part of a multifunctional complex involved in the activation of both NF-kappa B and JNK. Act1 fails to activate NF-kappa B in an IL-1-unresponsive mutant cell line in which all known signaling components are present, suggesting that it interacts with an unknown component in IL-1 signaling.