Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex
Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex
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DOI：
10.1007/s00294-005-0570-8
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发表时间：
2005-05-01
期刊：
影响因子：
2.5
通讯作者：
Chen, MY
中科院分区：
文献类型：
--
作者：
Ho, HL;Shiau, YS;Chen, MY
酿酒酵母TSC 11/AVO 3是编码TORC 2的一个组分的必需基因，TORC 2是酵母Tor 2 p的多蛋白复合物，还含有Lst 8 p、Avo 1 p和Avo 2 p。尽管已证实TORC 2组分之间存在物理关联，但对这些蛋白质作用的功能联系或细胞途径知之甚少。在这里，我们提出了将TSC 11的功能与细胞完整性调节联系起来的遗传数据。TSC 11不同区域携带温度敏感（TS）等位基因的突变体显示出细胞壁缺陷，这一点可通过特征性渗透稳定剂可修复的细胞溶解、对台盼蓝染色的敏感性和对细胞壁消化酶的敏感性来证明。剂量抑制分析确定了不同的基因组在拯救不同tsc 11（ts）突变体的表型。AVO 1抑制一类突变体，而活性PKC 1，AVO 2和SLM 1部分拯救另一类。我们的研究结果表明，TORC 2组件之间的功能连接，我们推测，Tsc 11 p发挥其功能，通过Pkc 1 p独立的机制，通过Avo 1 p介导，和Pkc 1 p依赖的机制，通过Avo 2 p和Slm 1 p介导。
Saccharomyces cerevisiae TSC11/AVO3 is an essential gene encoding one component of TORC2, a multi-protein complex of yeast Tor2p that also contains Lst8p, Avo1p, and Avo2p. Despite the proven physical association among TORC2 components, little is known about the functional linkage or cellular pathways these proteins act in. Here, we present genetic data linking the function of TSC11 to the regulation of cell integrity. Mutants carrying temperature-sensitive (ts) alleles in different regions of TSC11 displayed cell wall defects, evidenced by characteristic osmotic stabilizer-remediable cell lysis, susceptibility to trypan blue staining, and sensitivity to cell wall-digesting enzymes. Dosage suppression analysis identified different groups of genes in rescuing phenotypes of different tsc11(ts)mutants. AVO1 suppressed one class of mutants, whereas active PKC1, AVO2, and SLM1 partially rescued another. Our findings demonstrate functional connections among TORC2 components and we speculate that Tsc11p exerts its function via a Pkc1p-independent mechanism mediated through Avo1p, and a Pkc1p-dependent mechanism mediated through Avo2p and Slm1p.