A PCR based assay for detection and differentiation of African trypanosome species in blood
A PCR based assay for detection and differentiation of African trypanosome species in blood
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DOI：
10.1016/j.exppara.2005.03.014
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发表时间：
2005-09-01
影响因子：
2.1
通讯作者：
Welburn, S
中科院分区：
文献类型：
--
作者：
Cox, A;Tilley, A;Welburn, S
对大规模流行病学研究所需数量的锥虫感染血液样本进行直接 PCR 分析一直是个问题。目前识别和区分锥虫的方法通常需要几种物种特异性反应，其中许多依赖于小鼠传代样本来获得高质量的浓缩基因组 DNA。因此，重要的流行病学信息可能会在样品制备阶段丢失。在这里，我们报告了一种 PCR 方法，可以减少处理并提高现有筛选方法的灵敏度。 PCR 技术以编码小核糖体亚基的基因为目标，以识别和区分所有临床上重要的非洲锥虫物种和一些亚种。该方法比现有的筛查方法更经济、简单、灵敏，并能产生更详细的信息，从而使其成为大规模流行病学研究的可行工具。 (c) 2005 Elsevier Inc. 保留所有权利。
Direct PCR analysis of trypanosome infected blood samples in the quantities required for large scale epidemiological study has always been problematic. Current methods for identifying and differentiating trypanosomes typically require several species-specific reactions, many of which rely on mouse passaged samples to obtain quality concentrated genomic DNA. As a consequence important epidemiological information may be lost during the sample preparation stage. Here, we report a PCR methodology that reduces processing and improves on the sensitivity of present screening methods. The PCR technique targets the gene encoding the small ribosomal subunit in order to identify and differentiate all clinically important African trypanosome species and some subspecies. The method is more economical, simple, and sensitive than current screening methods, and yields more detailed information, thereby making it a viable tool for large-scale epidemiological studies. (c) 2005 Elsevier Inc. All rights reserved.