A pilot study on morphological compartmentalization and heterogeneity in the elasmobranch olfactory bulb.
A pilot study on morphological compartmentalization and heterogeneity in the elasmobranch olfactory bulb.
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软骨鱼嗅球形态区划和异质性的初步研究。
DOI：
10.1007/bf00191450
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Graziadei,PP
中科院分区：
文献类型：
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作者：
Dryer,L;Graziadei,PP
许多棘目鱼类的嗅球在形态上被细分为不同的单位或紧挨着嗅觉上皮的亚球。我们研究了两种鲨鱼和一种鱼的这种形态特征，以了解它对初级嗅觉投射到球茎的排列的影响。用顺行示踪方法，在体外(生物细胞素)和固定组织(DII)中，我们观察到嗅觉传入的直接分离投射到球上。将示踪剂应用于嗅觉上皮的外侧部分，导致染色仅限于该区域的球，而同样的示踪剂应用于上皮的内侧部分，导致内侧嗅球的染色。这些子球似乎是独立的解剖单位，每个都接受来自嗅板的输入。尼氏染色和髓鞘染色以及高尔基方法表明，亚鳞茎的细胞构筑与其他羊膜细胞的构筑没有本质的不同。然而，我们确实注意到了两种类型的二尖瓣细胞的存在，基于其树突树枝的形态。L细胞呈疏松的树突状树枝状，而T细胞呈密集的灌木状树枝状树枝状。两种类型的二尖瓣细胞均缺乏基树突状突起。
The olfactory bulb of many elasmobranch fishes is morphologically subdivided into distinct units or sub-bulbs immediately adjacent to the olfactory epithelium. We investigated this morphological feature in two species of shark and one species of ray in order to understand its impact on the arrangement of the primary olfactory projections onto the bulb. Using anterograde tracing methods in vitro (biocytin) as well as in fixed tissue (DiI), we observed a direct segregated projection of the olfactory afferents onto the bulb. Application of tracers to the lateral part of the olfactory epithelium resulted in staining restricted to the this region of the bulb, whereas the same tracers applied to the medial part of the epithelium resulted in staining of the medial olfactory bulb. The sub-bulbs appear to be individual anatomical units that each receive input from the olfactory lamellae. Nissl and myelin staining as well as the Golgi method show that the cytoarchitecture of the sub-bulbs is not substantially different from that of other anamniotes. However, we did note the existence of two types of mitral cell, based on the morphology of their dendritic arborization. Type L cells exhibit a loose dendritic arborization, whereas type T cells are characterized by a dense, bush-like dendritic arborization. Both types of mitral cells lack basal dendrites.