Depression of Myocardial Contractility in Rats by Free Fatty Acids during Hypoxia
Depression of Myocardial Contractility in Rats by Free Fatty Acids during Hypoxia
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缺氧时游离脂肪酸对大鼠心肌收缩力的抑制作用
DOI：
10.1161/01.res.26.4.439
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发表时间：
1970
影响因子：
20.1
通讯作者：
E. Sonnenblick
中科院分区：
文献类型：
--
作者：
A. Henderson;A. S. Most;W. Parmley;R. Gorlin;E. Sonnenblick
以大鼠乳头肌为实验材料，研究了葡萄糖(5 MM)、亚油酸(1~1.75 mM)、辛酸(0.5~1.75 mM)和对-4-烯酸(1~5 mM)对大鼠乳头肌在有氧、低氧和缺氧条件下力学性能的影响。缓冲液中含有0.3 mM白蛋白。游离脂肪酸(FFA)(1.0-1.75 mm)不会改变充氧条件下的机械性能。在低氧或缺氧状态下，FFA(0.5~1.75 mM)抑制心肌细胞的收缩，增加静息力；葡萄糖改善心肌细胞的力学性能，改善FFA的抑制作用。非代谢FFA戊烯酸的抑制作用与其他FFA相似。这表明，这种作用是由FFA或酰基辅酶A衍生物直接介导的，而不是它们的代谢产物，这可能是由于FFA的去污剂效应或FFA在低pH时存在的超过细胞内FFA结合量的钙结合所致。钙可以增强缺氧时的力量发展，这意味着肌丝收缩能力的降低不能完全归因于高能储存的减少。
Rat papillary muscles were used to study the influence of glucose (5 mM), linoleate (1 to 1.75 mM), octanoate (0.5 to 1.75 mM) and pent-4-enoic acid (1 to 5 mM) on mechanical performance under oxygenated, hypoxic and anoxic conditions. The buffer solution contained 0.3 mM albumin. Free fatty acids (FFA) (1.0 to 1.75 mM) did not alter mechanical performance under oxygenated conditions. During hypoxia or anoxia, FFA (0.5 to 1.75 mM) depressed contractility and increased resting force; glucose improved mechanical performance and modified the depressant effects of FFA. The depressant effect of the nonmetabolized FFA, pentenoic acid, was similar to that of other FFA. This suggests that the effect was mediated directly by FFA or acyl CoA derivatives rather than their metabolic products, and that it might be due to a detergent effect or calcium binding by FFA present in excess of intracellular FFA binding capacity at low pH. Force development during anoxia could be augmented by calcium, implying that the reduced ability of the myofilaments to contract could not be attributed entirely to a reduction of high energy stores.