Leech filamin and Tractin: markers for muscle development and nerve formation.
Leech filamin and Tractin: markers for muscle development and nerve formation.
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水蛭细丝蛋白和 Tractin：肌肉发育和神经形成的标记物。
DOI：
10.1002/neu.20035
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Johansen,Jorgen
中科院分区：
文献类型：
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作者：
Venkitaramani,DeepaV;Wang,Dong;Ji,Yun;Xu,Ying-Zhi;Ponguta,Liliana;Bock,Katie;Zipser,Birgit;Jellies,John;Johansen,KristenM;Johansen,Jorgen
The Lan3‐14 and Laz10‐1 monoclonal antibodies recognize a 400 kDa antigen that is specifically expressed by all muscle cells in leech. We show that the antigen recognized by both antibodies is a member of the filamin family of actin binding proteins. Leech filamin has two calponin homology domains and 35 filamin/ABP‐repeat domains. In addition, we used the Laz10‐1 antibody to characterize the development of the segmentally iterated dorsoventral flattener muscles. We demonstrate that the dorsoventral flattener muscle develops as three discrete bundles of myofibers and that CNS axons pioneering the DP nerve extend only along the middle bundle. Interestingly, the middle dorsoventral muscle anlage is associated with only non‐neuronal expression of the L1‐family cell adhesion molecule Tractin. This expression is transient and occurs at the precise developmental stages when DP nerve formation takes place. Based on these findings we propose that the middle dorsoventral muscle anlagen provides a substrate for early axonal outgrowth and nerve formation and that this function may be associated with differential expression of distinct cell adhesion molecules. © 2004 Wiley Periodicals, Inc. J Neurobiol 60: 369–380, 2004
