Cooperative effect of heparan sulfate and laminin mimetic peptide nanofibers on the promotion of neurite outgrowth
Cooperative effect of heparan sulfate and laminin mimetic peptide nanofibers on the promotion of neurite outgrowth
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DOI：
10.1016/j.actbio.2012.02.006
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发表时间：
2012-07-01
影响因子：
9.7
通讯作者：
Tekinay, Ayse B.
中科院分区：
文献类型：
--
作者：
Mammadov, Busra;Mammadov, Rashad;Tekinay, Ayse B.
细胞外基质含有丰富多样的信号，这些信号被细胞表面受体接收，通过调节细胞活动，如增殖、迁移和分化，从而促进细胞命运。来自细胞外基质的线索可用于开发材料，以指导细胞进入其期望的命运。神经细胞外基质（ECM）富含轴突生长诱导蛋白，并且通过模拟细胞环境中的这些容许元件，可以诱导神经分化以及神经突生长。在本文中，我们使用了一种合成的肽ESTA系统，它不仅可以模拟层粘连蛋白，轴突生长促进成分的神经ECM的活性，但也硫酸乙酰肝素蛋白多糖的活性，以诱导轴突发生。在我们的系统中使用的硫酸乙酰肝素模拟基团对生长因子具有亲和力，并诱导层粘连蛋白模拟肽纳米纤维的神经再生作用。具有硫酸乙酰肝素模拟物和层粘连蛋白衍生表位的自组装肽纳米纤维显著促进PC-12细胞的神经突起生长。此外，这些支架甚至在硫酸软骨素蛋白聚糖（CSPG）的存在下也是有效的，硫酸软骨素蛋白聚糖是中枢神经系统的主要抑制组分。在这些纳米纤维的存在下，细胞可以克服CSPG的抑制作用，并在肽支架上延伸神经突。(C)2012 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Extracellular matrix contains an abundant variety of signals that are received by cell surface receptors contributing to cell fate, via regulation of cellular activities such as proliferation, migration and differentiation. Cues from extracellular matrix can be used for the development of materials to direct cells into their desired fate. Neural extracellular matrix (ECM) is rich in axonal growth inducer proteins, and by mimicking these permissive elements in the cellular environment, neural differentiation as well as neurite outgrowth can be induced. In this paper, we used a synthetic peptide nanofiber system that can mimic not only the activity of laminin, an axonal growth-promoting constituent of the neural ECM, but also the activity of heparan sulfate proteoglycans in order to induce neuritogenesis. Heparan sulfate mimetic groups that were utilized in our system have an affinity to growth factors and induce the neuroregenerative effect of laminin mimetic peptide nanofibers. The self-assembled peptide nanofibers with heparan sulfate mimetic and laminin-derived epitopes significantly promoted neurite outgrowth by PC-12 cells. In addition, these scaffolds were even effective in the presence, of chondroitin sulfate proteoglycans (CSPGs), which are the major inhibitory components of the central nervous system. In the presence of these nanofibers, cells could overcome CSPG inhibitory effect and extend neurites on peptide nanofiber scaffolds. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.