Universal biosensor for detection of influenza virus
Universal biosensor for detection of influenza virus
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DOI：
10.1016/j.bios.2014.03.050
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发表时间：
2014-09-15
影响因子：
12.6
通讯作者：
Gromadzka, Beata
中科院分区：
文献类型：
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作者：
Nidzworski, Dawid;Pranszke, Paulina;Gromadzka, Beata
流感是一种由正粘病毒科病毒引起的人类传染性疾病。每年，流感病毒感染数百万人，并导致数十万人死亡。传统的诊断方法，如病毒培养和分离、抗原捕获免疫测定以及分子方法，对于流感病毒的检测并不充分。开发一种有效且能在不到一小时内快速检测病毒且具有高灵敏度的诊断方法，对全世界的研究人员来说是一项挑战。在此我们介绍一种新型的通用免疫传感器用于检测甲型流感病毒。通过使用电化学阻抗谱（EIS）以及将抗体直接附着在金电极上，该检测方法能够在相对较短的时间内以类似于分子方法的灵敏度检测病原体。通用抗M1抗体的应用使得能够检测甲型流感病毒的所有血清型。该传感器的简单设计有利于设备的小型化，并使其能够在对患者进行适当治疗之前的初次接触时用于常规诊断。（C）2014作者。由爱思唯尔出版集团出版。
Influenza is a contagious disease caught by humans caused by viruses belonging to the family Orthomyxoviridae. Each year, the influenza virus infects millions of people and kills hundreds of thousands of them. Traditional diagnostic methods, such as virus propagation and isolation, antigen capture immunoassays and molecular methods are not sufficient for the detection of the influenza virus. Development of a valid diagnostic assay for quick detection (in less than an hour) of the virus, with high sensitivity, is a challenge for researchers all over the world.Here we present a new, universal immunosensor for detection of the influenza A virus. By using electrochemical impedance spectroscopy (EIS) and direct attachment of antibodies to the gold electrode the assay allows detection of the pathogen with sensitivity similar to molecular methods in relatively short time. Application of universal anti-M1 antibodies allows detection of all serotypes of influenza A virus.The simple design of the sensor facilitates miniaturization of the device and its implementation for routine diagnostics during first contact with the patient, before applying a proper treatment. (C) 2014 The Authors. Published by Elsevier B.V.