Inversion Genotyping in theAnopheles gambiaeComplex Using High-Throughput Array and Sequencing Platforms
Inversion Genotyping in theAnopheles gambiaeComplex Using High-Throughput Array and Sequencing Platforms
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DOI：
10.1534/g3.120.401418
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发表时间：
2020-09-01
影响因子：
2.6
通讯作者：
Besansky, Nora J.
中科院分区：
文献类型：
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作者：
Love, R. Rebecca;Pombi, Marco;Besansky, Nora J.
染色体倒置多态性在疟疾媒介蚊子的冈比亚按蚊复合体中具有特殊的重要性，因为它们在当地适应和范围扩大中起作用。自然种群中反转的研究依赖于由专业细胞遗传学家进行的多染色体分析，这一过程受到训练有素的专家稀缺、低通量和限制性采样要求的限制。为了克服这一障碍，我们确定了与倒置状态（倒立或标准取向）高度相关的标签单核苷酸多态性（snp）。我们使用两种可选的高通量分子基因分型方法比较了标签snp的性能。同样960个个体的传统细胞遗传学核型分析。gambiaeandAn。取自西非布吉纳法索的科鲁兹蚊。我们表明，这两种分子方法产生可比的结果，并且任何一个表现良好或优于细胞遗传学方面的基因分型准确性。鉴于分子基因分型方法能够在不受蚊子性别或发育阶段限制的情况下以相对较低的成本大规模进行，通过标记snp进行分子基因分型有可能使染色体倒置在蚊子行为和持续适应中的作用的研究重新焕发活力。gambiaeandAn。coluzzito环境异质性。
Chromosomal inversion polymorphisms have special importance in theAnopheles gambiaecomplex of malaria vector mosquitoes, due to their role in local adaptation and range expansion. The study of inversions in natural populations is reliant on polytene chromosome analysis by expert cytogeneticists, a process that is limited by the rarity of trained specialists, low throughput, and restrictive sampling requirements. To overcome this barrier, we ascertained tag single nucleotide polymorphisms (SNPs) that are highly correlated with inversion status (inverted or standard orientation). We compared the performance of the tag SNPs using two alternative high throughput molecular genotyping approachesvs.traditional cytogenetic karyotyping of the same 960 individualAn. gambiaeandAn. coluzziimosquitoes sampled from Burkina Faso, West Africa. We show that both molecular approaches yield comparable results, and that either one performs as well or better than cytogenetics in terms of genotyping accuracy. Given the ability of molecular genotyping approaches to be conducted at scale and at relatively low cost without restriction on mosquito sex or developmental stage, molecular genotyping via tag SNPs has the potential to revitalize research into the role of chromosomal inversions in the behavior and ongoing adaptation ofAn. gambiaeandAn. coluzziito environmental heterogeneities.