Androgen-sensitivity of somata and dendrites of spinal nucleus of the bulbocavernosus (SNB) motoneurons in male C57BL6J mice
Androgen-sensitivity of somata and dendrites of spinal nucleus of the bulbocavernosus (SNB) motoneurons in male C57BL6J mice
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DOI：
10.1016/j.yhbeh.2006.10.003
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发表时间：
2007-02-01
影响因子：
3.5
通讯作者：
Jordan, Cynthia L.
中科院分区：
文献类型：
--
作者：
Zuloaga, Damian G.;Morris, John A.;Jordan, Cynthia L.
在成年大鼠中，雄激素调节球海绵体肌脊核（SNB）运动神经元的形态，包括其胞体的大小和树突的长度。关于雄激素是否对小鼠SNB运动神经元产生类似的影响，有相互矛盾的报道。我们阉割或假手术的C57BL6J小鼠在90天的年龄，30天后，注射霍乱毒素结合辣根过氧化物酶到球海绵体肌（标记SNB运动神经元）的一侧，并进入内在的足肌对侧（标记运动神经元的背外侧核（RDLN））。与假手术小鼠相比，去势小鼠的SNB胞体显著较小，而RDLN运动神经元的索马大小没有差异。C57BL6J小鼠的树突长度，估计在3维，成年去势后也显着下降。在大鼠中，雄激素通过雄激素受体（AR）直接作用于SNB运动神经元，控制索马的大小，几乎所有SNB运动神经元都含有AR。由于成年去势后C57BL6J小鼠的SNB胞体缩小，我们使用免疫细胞化学来表征SNB细胞以及RDLN和背外侧核（DLN）运动神经元中的AR表达。标记模式与之前在大鼠中观察到的相匹配：AR免疫反应性运动神经元的最高百分比位于SNB（98%），最低百分比位于RDLN（25%），中间数量位于DLN（78%）。这种AR标记模式与雄激素也直接作用于小鼠SNB运动神经元以调节小鼠索马大小的可能性一致。(c)2006年爱思唯尔公司All rights reserved.
In rats, androgens in adulthood regulate the morphology of motoneurons in the spinal nucleus of the bulbocavernosus (SNB), including the size of their somata and the length of their dendrites. There are conflicting reports about whether androgens exert similar influences on SNB motoneurons in mice. We castrated or sham-operated C57BL6J mice at 90 days of age and, thirty days later, injected cholera toxin conjugated horseradish peroxidase into the bulbocavernosus muscle (to label SNB motoneurons) on one side, and into intrinsic foot muscles contralaterally (to label motoneurons of the retrodorsolateral nucleus (RDLN)). Castrated mice had significantly smaller SNB somas compared to sham-operated mice while there were no differences in soma size of RDLN motoneurons. Dendritic length in C57BL6J mice, estimated in 3-dimensions, also decreased significantly after adult castration. In rats, androgens act directly through androgen receptors (AR) in SNB motoneurons to control soma size and nearly all SNB motoneurons contain AR. Since SNB somata in C57BL6J mice shrank after adult castration, we used immunocytochemistry to characterize AR expression in SNB cells as well as motoneurons in the RDLN and dorsolateral nucleus (DLN). A pattern of labeling matched that seen previously in rats: the highest percentage of AR-immunoreactive motoneurons are in the SNB (98%), the lowest in the RDLN (25%) and an intermediate number in the DLN (78%). This pattern of AR labeling is consistent with the possibility that androgens also act directly on SNB motoneurons in mice to regulate soma size in mice. (c) 2006 Elsevier Inc. All rights reserved.