Effects of Chronic and Acute Intraocular Pressure Elevation on Scotopic and Photopic Contrast Sensitivity in Mice.
Effects of Chronic and Acute Intraocular Pressure Elevation on Scotopic and Photopic Contrast Sensitivity in Mice.
复制标题
慢性和急性眼压升高对小鼠暗视和明视对比敏感度的影响。
DOI：
10.1167/iovs.16-19312
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发表时间：
2016
影响因子：
4.4
通讯作者：
Frankfort,BenjaminJ
中科院分区：
文献类型：
--
作者：
vanderHeijden,MeikeE;Shah,Priya;Cowan,CameronS;Yang,Zhuo;Wu,SamuelM;Frankfort,BenjaminJ
目的：比较眼内压（IOP）升高对小鼠暗视和明视对比敏感度的影响。方法：我们通过注射聚苯乙烯珠或通过注射高粘性透明质酸钠来慢性升高野生型小鼠的IOP。一些患有慢性IOP升高的患眼局部使用0.1%酒石酸溴莫尼定或1%布林佐胺进行治疗。暗视和明视对比敏感度在多个时间点的峰值时空频率进行了评估，与一个既定的视动技术。视网膜神经节细胞（RGC）计数用针对III类β-微管蛋白的抗体测定。IOP水平，RGC计数，和暗视或明视对比敏感度之间的相关性performed.Results：6周的IOP升高引起了广泛的减少明视对比敏感度和优先减少暗视对比敏感度在峰值时空频率。布林佐胺而非溴莫尼定的给药导致累积IOP显著降低，而溴莫尼定而非布林佐胺可预防RGC损失。溴莫尼定和布林佐胺都能防止对比敏感度的丧失，但溴莫尼定在更早的时间点和更广泛的光照条件下都能做到这一点。无论是慢性或急性眼压升高，暗视对比敏感度的影响最显着的眼压水平，而不是RGC计数，而明视对比敏感度的组合factors.Conclusions的影响：这是可能的，暗视特定的视网膜回路改变优先眼压升高，暗视对比敏感度的变化将有助于青光眼检测。溴莫尼定似乎通过IOP非依赖性机制预防RGC丢失。
Purpose: To compare the impact of intraocular pressure (IOP) elevation on scotopic and photopic contrast sensitivity in mice.Methods: We chronically elevated the IOP of wild-type mice via injection of polystyrene beads or acutely via injection of highly cohesive sodium hyaluronate. Some eyes with chronically elevated IOP were treated with either topical brimonidine tartrate 0.1% or brinzolamide 1%. Scotopic and photopic contrast sensitivity was assessed at peak spatiotemporal frequencies at multiple time points, with an established optokinetic technique. Retinal ganglion cell (RGC) counts were determined with an antibody to class III beta-tubulin. Correlations among IOP level, RGC count, and scotopic or photopic contrast sensitivity were performed.Results: Six weeks of IOP elevation caused a generalized reduction of photopic contrast sensitivity and a preferential reduction of scotopic contrast sensitivity at peak spatiotemporal frequencies. The administration of brinzolamide but not brimonidine caused a significant reduction in cumulative IOP, whereas brimonidine, but not brinzolamide, prevented RGC loss. Both brimonidine and brinzolamide prevented contrast sensitivity loss, but brimonidine did so at earlier time points and across a wider range of lighting conditions. Following either chronic or acute IOP elevation, scotopic contrast sensitivity was impacted most prominently by IOP level and not by RGC count, while photopic contrast sensitivity was impacted by a combination of factors.Conclusions: It is possible that scotopic-specific retinal circuitry is altered preferentially by IOP elevation, and that changes in scotopic contrast sensitivity will assist with glaucoma detection. Brimonidine appears to prevent RGC loss via an IOP-independent mechanism.