Age related optic nerve axonal loss in adult Brown Norway rats
Age related optic nerve axonal loss in adult Brown Norway rats
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DOI：
10.1016/j.exer.2004.12.021
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发表时间：
2005-06-01
影响因子：
3.4
通讯作者：
Morrison, JC
中科院分区：
文献类型：
--
作者：
Cepurna, WO;Kayton, RJ;Morrison, JC
使用透射电子显微镜 (TEM) 检查年龄对成年棕色挪威大鼠（5-31 个月大）（n=29）视神经轴突数量和形态的影响。通过手动计算代表视神经横截面 60% 的区域中的每个轴突。我们发现年龄和轴突计数之间存在显着的负相关（R-2 = 0.18，P < 0.05）。然而，当忽略最年长的动物时，这种关系不再具有统计学意义。同时，自发退化轴突的比例呈指数级增长（R-2 = 0.79 P < 0.05），31 个月组的退化明显多于 5 个月大的动物（ANOVA，P < 0.05）。这项研究表明，使用定量 TEM 方法，在棕色挪威大鼠成年后的大部分时间里，视神经轴突数量相对恒定， 青光眼研究中越来越受欢迎的菌株。随衰老而发生的总轴突损失大大低于其他菌株报道的损失。直到生命的最后几个月，轴突数量的减少和轴突变性的速度才出现显着改变，这无法支持一些研究的结论，即成年大鼠的视神经轴突损失是线性的。然而。他们确实同意在大鼠中进行的其他研究以及在非人类灵长类动物眼睛中进行的类似研究，该研究得出的结论是视神经和视网膜的衰老变化遵循复杂的模式。因此，在对大鼠实验性青光眼视神经损伤的过程和病理生理学进行建模时，必须考虑动物年龄的影响。 (c) 2005 Elsevier Ltd. 保留所有权利。
The effect of age on the number and morphology of optic nerve axons in adult Brown Norway rats (5-31 months old) (n=29) was examined using transmission electron microscopy (TEM). By manually counting every axon in areas representing 60% of the optic nerve cross-section. we found a significant negative correlation between age and axon count (R-2=0.18, P < 0.05). However, when the oldest animals were omitted, the relationship was no longer statistically significant. Simultaneously, the proportion of spontaneously degenerating axons increased at an exponential rate (R-2=0.79 P < 0.05), with significantly more degeneration in the 31-month group than in 5-month-old animals (ANOVA, P < 0.05).This study demonstrates, using quantitative TEM methods, that optic nerve axonal numbers are relatively constant throughout the majority of the adult life of the Brown Norway rat, an increasingly popular strain for glaucoma research. Total axonal loss with aging is substantially less than that reported for other strains. The reduction in axonal numbers and the rate of axonal degeneration do not appear significantly altered until the last few months of life, failing to support some studies that have concluded that optic nerve axon loss in adult rats is linear. However. they do agree with other studies in the rat, and a similar study performed in non-human primate eyes, that concluded that aging changes in the optic nerve and retina follow a complex pattern. Therefore, the impact of animal age must be considered when modeling the course and pathophysiology of experimental glaucomatous optic nerve damage in rats. (c) 2005 Elsevier Ltd. All rights reserved.