OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells.
OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells.
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OMIP-008：测量人类 T 细胞的 Th1 和 Th2 细胞因子多功能性。
DOI：
10.1002/cyto.a.22035
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Albertini,MarkR
中科院分区：
文献类型：
--
作者：
Zuleger,CindyL;Albertini,MarkR
目的和适当的样品类型优化该组以评估对来自黑素瘤患者的各种肿瘤抗原的CD 4+和CD 8 + T细胞应答。在来自黑素瘤患者的外周血单核细胞（PBMC）的单细胞衍生的T细胞分离物（SCD-T）和T细胞系、来自黑素瘤患者的肿瘤环境的T细胞系以及新鲜的和冷冻保存的PBMC（健康供体）上测试了该组。染色可以在96孔板中进行，以实现高通量。
Purpose and Appropriate Sample Types This panel was optimized to assess CD4+ and CD8+ T cell responses to various tumor antigens from melanoma patients. The panel was tested on single-cell derived T cell isolates (SCD-T) and T cell lines derived from peripheral blood mononuclear cells (PBMC) from melanoma patients, T cell lines from the tumor environment of melanoma patients, and fresh and cryopreserved PBMC (healthy donors). Staining can be performed in 96-well plates for high-throughput.