HLA CLASS-I (BG) ANTIGENS ON RED-CELLS OF SLE PATIENTS - A SEROLOGICAL STUDY WITH POLYCLONAL AND MONOCLONAL-ANTIBODIES
HLA CLASS-I (BG) ANTIGENS ON RED-CELLS OF SLE PATIENTS - A SEROLOGICAL STUDY WITH POLYCLONAL AND MONOCLONAL-ANTIBODIES
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DOI：
10.1111/j.1423-0410.1989.tb02038.x
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发表时间：
1989-01-01
期刊：
影响因子：
2.7
通讯作者：
BOTTO, M
中科院分区：
文献类型：
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作者：
GILES, CM;DARKE, C;BOTTO, M
许多系统性红斑狼疮患者红细胞上HLA-I类抗原（Bg）的增加使其HLA-B型与其对妊娠单独刺激的淋巴细胞毒性抗-HLA-B血清的血凝反应性之间存在显著的相关性。因此，这些RBC上的I类表达与经典而非经典的I类基因产物有关。通过针对重多肽链和β 2-微球蛋白（β 2 m）上的表位的单克隆抗体（MAb）对RBC的I类表达的研究表明存在完整的细胞外结构。氯喹在从RBC“剥离HLA”中的特异性作用被认为支持HLA I类从血浆中吸附的概念。然而，从我们的数据，我们得出结论，HLA I类是一种内在的膜组件。我们认为氯喹的作用是单独去除β 2 m，这阻止了正常的I类表达，并且还导致I类重链的构象变化，但是它不能去除膜结合的重链。
The enhanced HLA class I (Bg) on red blood cells (RBC) of many patients with systemic lupus erythematosus has allowed a significant correlation to be made between their HLA-B types and haemagglutination reactivity with lymphocytotoxic anti-HLA-B sera stimulated by pregnancy alone. Therefore the class I expression on these RBC relates to classical, rather than non-classical, class I gene products. Studies of class I expression of RBC by means of monoclonal antibodies (MAb) to epitopes on the heavy polypeptide chain and .beta.2-microglobulin (.beta.2m) have suggested that the complete extracellular structure is present. The specific effect of chloroquine in ''stripping HLA'' from RBC had been assumed to support the concept that HLA class I was adsorbed from plasma. However, from our data, we conclude that HLA class I is an intrinsic membrane component. We suggest that the action of chloroquine is to remove .beta.2m alone, which prevents normal class I expression and also results in conformational changes to the class I heavy chain, but that it is not capable of removing the membrane-bound heavy chain.